1fat: Difference between revisions

Revision as of 16:06, 2 May 2008

PHYTOHEMAGGLUTININ-L

OverviewOverview

The structure of phytohemagglutinin-L (PHA-L), a leucoagglutinating seed lectin from Phaseolus vulgaris, has been solved with molecular replacement using the coordinates of lentil lectin as model, and refined at a resolution of 2.8 A. The final R-factor of the structure is 20.0%. The quaternary structure of the PHA-L tetramer differs from the structures of the concanavalin A and peanut lectin tetramers, but resembles the structure of the soybean agglutinin tetramer. PHA-L consists of two canonical legume lectin dimers that pack together through the formation of a close contact between two beta-strands. Of the two covalently bound oligosaccharides per monomer, only one GlcNAc residue per monomer is visible in the electron density. In this article we describe the structure of PHA-L, and we discuss the putative position of the high affinity adenine-binding site present in a number of legume lectins. A comparison with transthyretin, a protein that shows a remarkable resemblance to PHA-L, gives further ground to our proposal.

About this StructureAbout this Structure

1FAT is a Single protein structure of sequence from Phaseolus vulgaris. Full crystallographic information is available from OCA.

ReferenceReference

The crystallographic structure of phytohemagglutinin-L., Hamelryck TW, Dao-Thi MH, Poortmans F, Chrispeels MJ, Wyns L, Loris R, J Biol Chem. 1996 Aug 23;271(34):20479-85. PMID:8702788 Page seeded by OCA on Fri May 2 16:06:48 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1fat.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1fat", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=CAA:Ca+Binding+Site+In+Chain+A'>CAA</scene>, <scene name='pdbsite=CAB:Ca+Binding+Site+In+Chain+B'>CAB</scene>, <scene name='pdbsite=CAC:Ca+Binding+Site+In+Chain+C'>CAC</scene>, <scene name='pdbsite=CAD:Ca+Binding+Site+In+Chain+D'>CAD</scene>, <scene name='pdbsite=MNA:Mn+Binding+Site+In+Chain+A'>MNA</scene>, <scene name='pdbsite=MNB:Mn+Binding+Site+In+Chain+B'>MNB</scene>, <scene name='pdbsite=MNC:Mn+Binding+Site+In+Chain+C'>MNC</scene> and <scene name='pdbsite=MND:Mn+Binding+Site+In+Chain+D'>MND</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1fat|  PDB=1fat  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fat OCA], [http://www.ebi.ac.uk/pdbsum/1fat PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fat RCSB]</span>
-}}
 '''PHYTOHEMAGGLUTININ-L'''
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 [[Category: Hamelryck, T.]]
 [[Category: Loris, R.]]
-[[Category: glycoprotein]]
+[[Category: Glycoprotein]]
-[[Category: lectin]]
+[[Category: Lectin]]
-[[Category: plant defense protein]]
+[[Category: Plant defense protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:06:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:19:19 2008''