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{{STRUCTURE_1f89| PDB=1f89 | SCENE= }} | |||
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'''Crystal structure of Saccharomyces cerevisiae Nit3, a member of branch 10 of the nitrilase superfamily''' | '''Crystal structure of Saccharomyces cerevisiae Nit3, a member of branch 10 of the nitrilase superfamily''' | ||
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[[Category: Studier, F W.]] | [[Category: Studier, F W.]] | ||
[[Category: Swaminathan, S.]] | [[Category: Swaminathan, S.]] | ||
[[Category: | [[Category: Dimer]] | ||
[[Category: | [[Category: Four layer sandwich]] | ||
[[Category: | [[Category: New york structural genomix research consortium]] | ||
[[Category: | [[Category: Nitrilase]] | ||
[[Category: | [[Category: Nysgxrc]] | ||
[[Category: | [[Category: Protein structure initiative]] | ||
[[Category: | [[Category: Psi]] | ||
[[Category: | [[Category: Structural genomic]] | ||
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Revision as of 16:01, 2 May 2008
Crystal structure of Saccharomyces cerevisiae Nit3, a member of branch 10 of the nitrilase superfamily
OverviewOverview
The crystal structure of a yeast hypothetical protein with sequence similarity to CN hydrolases has been determined to 2.4 A resolution by the multiwavelength anomalous dispersion (MAD) method. The protein folds as a four-layer alphabetabetaalpha sandwich and exists as a dimer in the crystal and in solution. It was selected in a structural genomics project as representative of CN hydrolases at a time when no structures had been determined for members of this family. Structures for two other members of the family have since been reported and the three proteins have similar topology and dimerization modes, which are distinct from those of other alphabetabetaalpha proteins whose structures are known. The dimers form an unusual eight-layer alphabetabetaalpha:alphabetabetaalpha structure. Although the precise enzymatic reactions catalyzed by the yeast protein are not known, considerable information about the active site may be deduced from conserved sequence motifs, comparative biochemical information, and comparison with known structures of hydrolase active sites. As with serine hydrolases, the active-site nucleophile (cysteine in this case) is positioned on a nucleophile elbow.
About this StructureAbout this Structure
1F89 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a putative CN hydrolase from yeast., Kumaran D, Eswaramoorthy S, Gerchman SE, Kycia H, Studier FW, Swaminathan S, Proteins. 2003 Aug 1;52(2):283-91. PMID:12833551 Page seeded by OCA on Fri May 2 16:01:08 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Saccharomyces cerevisiae
- Single protein
- Burley, S K.
- Eswaramoorthy, S.
- Kumaran, D.
- NYSGXRC, New York Structural GenomiX Research Consortium.
- Studier, F W.
- Swaminathan, S.
- Dimer
- Four layer sandwich
- New york structural genomix research consortium
- Nitrilase
- Nysgxrc
- Protein structure initiative
- Psi
- Structural genomic