1qil: Difference between revisions
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<StructureSection load='1qil' size='340' side='right'caption='[[1qil]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='1qil' size='340' side='right'caption='[[1qil]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1qil]] is a 3 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1qil]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QIL FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qil OCA], [https://pdbe.org/1qil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qil RCSB], [https://www.ebi.ac.uk/pdbsum/1qil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qil ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[ | [[https://www.uniprot.org/uniprot/TSST_STAAU TSST_STAAU]] Responsible for the symptoms of toxic shock syndrome. | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Revision as of 12:57, 15 September 2021
INACTIVE MUTANT TOXIC SHOCK SYNDROME TOXIN-1 AT 2.5 AINACTIVE MUTANT TOXIC SHOCK SYNDROME TOXIN-1 AT 2.5 A
Structural highlights
Function[TSST_STAAU] Responsible for the symptoms of toxic shock syndrome. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedToxic shock syndrome toxin-1 (TSST-1) is one of a family of staphylococcal exotoxins recognized as microbial superantigens. The toxin plays a dominant role in the genesis of toxic shock in humans through a massive activation of the immune system. This potentially lethal illness occurs as a result of the interaction of TSST-1 with a significant proportion of the T-cell repertoire. TSST-1, like other superantigens, can bind directly to class II major histocompatibility (MHC class II) molecules prior to its interaction with entire families of V beta chains of the T-cell receptor (TCR). The three-dimensional structure of a mutant (His-135-Ala) TSST-1 was compared with the structure of the native (wild-type) TSST-1 at 2.5 A resolution. The replacement of His 135 of TSST-1 with an Ala residue results in the loss of T-cell mitogenicity and toxicity in experimental animals. This residue, postulated to be directly involved in the toxin-TCR interactions, is located on the major helix alpha 2, which forms the backbone of the molecule and is exposed to the solvent. In the molecular structure of the mutant toxin, the helix alpha 2 remains unaltered, but the His to Ala modification causes perturbations on the neighboring helix alpha 1 by disrupting helix-helix interactions. Thus, the effects on TCR binding of the His 135 residue could actually be mediated, wholly or in part, by the alpha 1 helix. Crystal structure of a biologically inactive mutant of toxic shock syndrome toxin-1 at 2.5 A resolution.,Papageorgiou AC, Quinn CP, Beer D, Brehm RD, Tranter HS, Bonventre PF, Acharya KR Protein Sci. 1996 Aug;5(8):1737-41. PMID:8844860[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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