7rm5: Difference between revisions
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==MicroED structure of the human adenosine receptor at 2.8A== | ==MicroED structure of the human adenosine receptor at 2.8A== | ||
<StructureSection load='7rm5' size='340' side='right'caption='[[7rm5]]' scene=''> | <StructureSection load='7rm5' size='340' side='right'caption='[[7rm5]], [[Resolution|resolution]] 2.79Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RM5 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7rm5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RM5 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rm5 OCA], [https://pdbe.org/7rm5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rm5 RCSB], [https://www.ebi.ac.uk/pdbsum/7rm5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rm5 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZMA:4-{2-[(7-AMINO-2-FURAN-2-YL[1,2,4]TRIAZOLO[1,5-A][1,3,5]TRIAZIN-5-YL)AMINO]ETHYL}PHENOL'>ZMA</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ADORA2A, ADORA2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rm5 OCA], [https://pdbe.org/7rm5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rm5 RCSB], [https://www.ebi.ac.uk/pdbsum/7rm5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rm5 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[https://www.uniprot.org/uniprot/AA2AR_HUMAN AA2AR_HUMAN]] Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
G protein-coupled receptors (GPCRs), or seven-transmembrane receptors, are a superfamily of membrane proteins that are critically important to physiological processes in the human body. Determining high-resolution structures of GPCRs without bound cognate signaling partners, such as a G protein, requires crystallization in lipidic cubic phase (LCP). GPCR crystals grown in LCP are often too small for traditional X-ray crystallography. These microcrystals are ideal for investigation by microcrystal electron diffraction (MicroED), but the gel-like nature of LCP makes traditional approaches to MicroED sample preparation insurmountable. Here, we show that the structure of a human A2A adenosine receptor can be determined by MicroED after converting the LCP into the sponge phase followed by focused ion-beam milling. We determined the structure of the A2A adenosine receptor to 2.8-A resolution and resolved an antagonist in its orthosteric ligand-binding site, as well as four cholesterol molecules bound around the receptor. This study lays the groundwork for future structural studies of lipid-embedded membrane proteins by MicroED using single microcrystals that would be impossible with other crystallographic methods. | |||
MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP.,Martynowycz MW, Shiriaeva A, Ge X, Hattne J, Nannenga BL, Cherezov V, Gonen T Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2106041118. doi:, 10.1073/pnas.2106041118. PMID:34462357<ref>PMID:34462357</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7rm5" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bacillus coli migula 1895]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cherezov V]] | [[Category: Cherezov, V]] | ||
[[Category: Ge X]] | [[Category: Ge, X]] | ||
[[Category: Gonen T]] | [[Category: Gonen, T]] | ||
[[Category: Hattne J]] | [[Category: Hattne, J]] | ||
[[Category: Martynowycz | [[Category: Martynowycz, M W]] | ||
[[Category: Nannenga | [[Category: Nannenga, B L]] | ||
[[Category: Shiriaeva A]] | [[Category: Shiriaeva, A]] | ||
[[Category: Membrane protein]] | |||