1f2i: Difference between revisions

Revision as of 15:48, 2 May 2008

COCRYSTAL STRUCTURE OF SELECTED ZINC FINGER DIMER BOUND TO DNA

OverviewOverview

Protein-protein interactions often play a crucial role in stabilizing protein-DNA complexes and thus facilitate site-specific DNA recognition. We have worked to incorporate such protein-protein contacts into our design and selection strategies for short peptide extensions that promote cooperative binding of zinc finger proteins to DNA. We have determined the crystal structure of one of these fusion protein-DNA complexes. The selected peptide extension was found to mediate dimerization by reaching across the dyad axis and contacting a hydrophobic patch on the surface of the zinc finger bound to the adjacent DNA site. The peptide-zinc finger protein interactions observed in this structure are similar to those of some homeodomain heterodimers. We also find that the region of the zinc finger surface contacted by the selected peptide extension corresponds to surfaces that also make key interactions in the zinc finger proteins GLI and SWI5.

About this StructureAbout this Structure

1F2I is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Selected peptide extension contacts hydrophobic patch on neighboring zinc finger and mediates dimerization on DNA., Wang BS, Grant RA, Pabo CO, Nat Struct Biol. 2001 Jul;8(7):589-93. PMID:11427887 Page seeded by OCA on Fri May 2 15:48:46 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1f2i.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1f2i |SIZE=350|CAPTION= <scene name='initialview01'>1f2i</scene>, resolution 2.35&Aring;
+The line below this paragraph, containing "STRUCTURE_1f2i", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= GENE FOR ZIF12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
+-->
-|DOMAIN=
+{{STRUCTURE_1f2i|  PDB=1f2i  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f2i OCA], [http://www.ebi.ac.uk/pdbsum/1f2i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f2i RCSB]</span>
-}}
 '''COCRYSTAL STRUCTURE OF SELECTED ZINC FINGER DIMER BOUND TO DNA'''
@@ Line 28: / Line 25: @@
 [[Category: Pabo, C O.]]
 [[Category: Wang, B S.]]
-[[Category: cooperativity]]
+[[Category: Cooperativity]]
-[[Category: dimer]]
+[[Category: Dimer]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
-[[Category: zinc finger]]
+[[Category: Zinc finger]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:48:46 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:14:26 2008''