Human lactoferrin: Difference between revisions
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The structure of LF<sub>N</sub> undergoes a dramatic conformational change upon iron binding. Upon iron binding, the two domains of LF<sub>N</sub> undergo a rigid 54.1º rotation about a <scene name='Sandbox_Reserved_302/Hinge/1'>screw axis</scene> that passes through Thr90 and Pro251.<ref name="gerstein">PMID:8230220</ref> | The structure of LF<sub>N</sub> undergoes a dramatic conformational change upon iron binding. Upon iron binding, the two domains of LF<sub>N</sub> undergo a rigid 54.1º rotation about a <scene name='Sandbox_Reserved_302/Hinge/1'>screw axis</scene> that passes through Thr90 and Pro251.<ref name="gerstein">PMID:8230220</ref> | ||
<jmol> | To visualize the <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script>load files "=1CB6" "=1LCF";model 0;cartoon only;domain2 = {(92-249 or 1092-1249)}; | <script>load files "=1CB6" "=1LCF";model 0;cartoon only;domain2 = {(92-249 or 1092-1249)};select domain2;color red; | ||
structures = [{1.1}, {2.1}]; | structures = [{1.1}, {2.1}]; | ||
domains = [[{not domain2},{not domain2}, {alpha and not domain2 and not altloc="B"}], [{domain2}, {(91,1091,250,1250) and *.CA}, {alpha and domain2 and not altloc="B"}],]; | domains = [[{not domain2},{not domain2}, {alpha and not domain2 and not altloc="B"}], [{domain2}, {(91,1091,250,1250) and *.CA}, {alpha and domain2 and not altloc="B"}],]; | ||
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<text>domain motion</text> | <text>domain motion</text> | ||
</jmolLink> | </jmolLink> | ||
</jmol> | </jmol>, we first load the two structures and superimpose them. You can choose a viewing orientation before pressing the morph button, which will visualize the conformational change from structure 1CB6 to 1LCF and back again. | ||
<jmol> | <jmol> | ||