Electron cryomicroscopy: Difference between revisions
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[http://tinyurl.com/method-vs-resolution tinyurl.com/method-vs-resolution].</ref>. For comparison, the median resolution of X-ray crystallographic entries in the PDB has been 2.0 Å for many years<ref name="mvr" />. | [http://tinyurl.com/method-vs-resolution tinyurl.com/method-vs-resolution].</ref>. For comparison, the median resolution of X-ray crystallographic entries in the PDB has been 2.0 Å for many years<ref name="mvr" />. | ||
In 2015, Cheng, Grigorieff, Penczek & Walz concluded: <blockquote> | In 2015, Cheng, Grigorieff, Penczek & Walz<ref name="primer" /> concluded: <blockquote> | ||
"''Resolution'' in single-particle EM is ... a somewhat arbitrarily chosen cut-off level ...." "... it is the opinion of the authors that there is currently no real ''gold standard'' procedure for structure refinement and resolution estimation of an EM map."<ref name="primer" />. | "''Resolution'' in single-particle EM is ... a somewhat arbitrarily chosen cut-off level ...." "... it is the opinion of the authors that there is currently no real ''gold standard'' procedure for structure refinement and resolution estimation of an EM map."<ref name="primer" />. | ||
</blockquote> | </blockquote> | ||