Electron cryomicroscopy: Difference between revisions
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Cryo-EM "has the advantage of recording images containing both amplitude and phase information, so there is no phase problem as in [X-ray] crystallography"<ref name="rosenthal2019">PMID: 30713698</ref>. | Cryo-EM "has the advantage of recording images containing both amplitude and phase information, so there is no phase problem as in [X-ray] crystallography"<ref name="rosenthal2019">PMID: 30713698</ref>. | ||
Electrons are diffracted by the charges in the sample, in contrast to X-rays that are diffracted by electron density. Consequently, EM produces an "electric potential map"<ref name="wang2016">PMID: 27706888</ref> or | Electrons are diffracted by the charges in the sample, in contrast to X-rays that are diffracted by electron density. Consequently, EM produces an "electric potential map"<ref name="wang2016">PMID: 27706888</ref> or "electron potential map"<ref name="rosenthal2019" />. Electron densities are all positive, while EM densities can be positive or negative<ref name="wang2016" />. | ||
==Temperatures (B factors)== | ==Temperatures (B factors)== | ||