Electron cryomicroscopy: Difference between revisions
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The result of a cryo-EM experiment is a density map. Just as for X-ray diffraction, it is then necessary to fit an atomic model optimally into the map. | The result of a cryo-EM experiment is a density map. Just as for X-ray diffraction, it is then necessary to fit an atomic model optimally into the map. | ||
Electrons are diffracted by the charges in the sample, in contrast to X-rays that are diffracted by electron density. Consequently, EM produces an "electric potential map"<ref>PMID: 27706888</ref> or .... Electron densities are all positive, while EM densities can be positive or negative. | Electrons are diffracted by the charges in the sample, in contrast to X-rays that are diffracted by electron density. Consequently, EM produces an "electric potential map"<ref name="wang2016">PMID: 27706888</ref> or .... Electron densities are all positive, while EM densities can be positive or negative<ref name="wang2016" />. | ||
==Temperatures (B factors)== | ==Temperatures (B factors)== | ||