1eyu: Difference between revisions

Revision as of 15:41, 2 May 2008

HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6

OverviewOverview

Restriction endonucleases differ in their use of metal cofactors despite having remarkably similar folds for their catalytic regions. To explore this, we have characterized the interaction of endonuclease PvuII with the catalytically incompetent cation Ca(2+). The structure of a glutaraldehyde-crosslinked crystal of the endonuclease PvuII-DNA complex, determined in the presence of Ca(2+) at a pH of approximately 6.5, supports a two-metal mechanism of DNA cleavage by PvuII. The first Ca(2+) position matches that found in all structurally examined endonucleases, while the second position is similar to that of EcoRV but is distinct from that of BamHI and BglI. The location of the second metal in PvuII, unlike that in BamHI/BglI, permits no direct interaction between the second metal and the O3' oxygen leaving group. However, the interactions between the DNA scissile phosphate and the metals, the first metal and the attacking water, and the attacking water and DNA are the same in PvuII as they are in the two-metal models of BamHI and BglI, but are distinct from the proposed three-metal or the two-metal models of EcoRV.

About this StructureAbout this Structure

1EYU is a Single protein structure of sequence from Proteus vulgaris. Full crystallographic information is available from OCA.

ReferenceReference

PvuII endonuclease contains two calcium ions in active sites., Horton JR, Cheng X, J Mol Biol. 2000 Jul 28;300(5):1049-56. PMID:10903853 Page seeded by OCA on Fri May 2 15:41:02 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1eyu.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1eyu", creates the "Structure Box" on the page.
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1eyu|  PDB=1eyu  |  SCENE=  }}
-|RELATEDENTRY=[[3pvi|3PVI]], [[2pvi|2PVI]], [[1pvi|1PVI]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyu OCA], [http://www.ebi.ac.uk/pdbsum/1eyu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eyu RCSB]</span>
-}}
 '''HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6'''
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 [[Category: Cheng, X.]]
 [[Category: Horton, J R.]]
-[[Category: endonuclease type ii]]
+[[Category: Endonuclease type ii]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
-[[Category: restriction enzyme]]
+[[Category: Restriction enzyme]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:41:02 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:12:24 2008''