1evl: Difference between revisions

Revision as of 15:34, 2 May 2008

CRYSTAL STRUCTURE OF A TRUNCATED FORM OF THREONYL-TRNA SYNTHETASE WITH A THREONYL ADENYLATE ANALOG

OverviewOverview

Accurate translation of the genetic code depends on the ability of aminoacyl-tRNA synthetases to distinguish between similar amino acids. In order to investigate the basis of amino acid recognition and to understand the role played by the zinc ion present in the active site of threonyl-tRNA synthetase, we have determined the crystal structures of complexes of an active truncated form of the enzyme with a threonyl adenylate analog or threonine. The zinc ion is directly involved in threonine recognition, forming a pentacoordinate intermediate with both the amino group and the side chain hydroxyl. Amino acid activation experiments reveal that the enzyme shows no activation of isosteric valine, and activates serine at a rate 1,000-fold less than that of cognate threonine. This study demonstrates that the zinc ion is neither strictly catalytic nor structural and suggests how the zinc ion ensures that only amino acids that possess a hydroxyl group attached to the beta-position are activated.

About this StructureAbout this Structure

1EVL is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Zinc ion mediated amino acid discrimination by threonyl-tRNA synthetase., Sankaranarayanan R, Dock-Bregeon AC, Rees B, Bovee M, Caillet J, Romby P, Francklyn CS, Moras D, Nat Struct Biol. 2000 Jun;7(6):461-5. PMID:10881191 Page seeded by OCA on Fri May 2 15:34:09 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1evl.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1evl |SIZE=350|CAPTION= <scene name='initialview01'>1evl</scene>, resolution 1.55&Aring;
+The line below this paragraph, containing "STRUCTURE_1evl", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=TSB:5&#39;-O-(N-(L-THREONYL)-SULFAMOYL)ADENOSINE'>TSB</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Threonine--tRNA_ligase Threonine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.3 6.1.1.3] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1evl|  PDB=1evl  |  SCENE=  }}
-|RELATEDENTRY=[[1qf6|1QF6]], [[1evk|1EVK]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1evl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1evl OCA], [http://www.ebi.ac.uk/pdbsum/1evl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1evl RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF A TRUNCATED FORM OF THREONYL-TRNA SYNTHETASE WITH A THREONYL ADENYLATE ANALOG'''
@@ Line 30: / Line 27: @@
 [[Category: Rees, B.]]
 [[Category: Sankaranarayanan, R.]]
-[[Category: adenylate analog]]
+[[Category: Adenylate analog]]
-[[Category: amino acid recognition]]
+[[Category: Amino acid recognition]]
-[[Category: deletion mutant]]
+[[Category: Deletion mutant]]
-[[Category: trna-synthetase]]
+[[Category: Trna-synthetase]]
-[[Category: zinc ion]]
+[[Category: Zinc ion]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:34:09 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:10:28 2008''