1ev6: Difference between revisions

Revision as of 15:33, 2 May 2008

Structure of the monoclinic form of the M-cresol/insulin R6 hexamer

OverviewOverview

The structures of three R(6) human insulin hexamers have been determined. Crystals of monoclinic m-cresol-insulin, monoclinic resorcinol-insulin and rhombohedral m-cresol-insulin diffracted to 1. 9, 1.9 and 1.78 A, respectively, and have been refined to residuals of 0.195, 0.179 and 0.200, respectively. In all three structures, a phenolic derivative is found to occupy the phenolic binding site, where it forms hydrogen bonds to the carbonyl O atom of CysA6 and the N atom of CysA11. Two additional phenolic derivative binding sites were identified within or between hexamers. The structures of all three hexamers are nearly identical, although a large displacement of the N-terminus of one B chain in both monoclinic structures results from coordination to a sodium ion which is located between symmetry-related hexamers. Other minor differences in structure arise from differences in packing in the monoclinic cell compared with the rhombohedral cell. Based upon the differences in conformation of the GluB13 side chains in T(6), T(3)R(f)(3) and R(6) hexamers, the deprotonation of these side chains appears to be associated with the T-->R conformational transition.

About this StructureAbout this Structure

1EV6 is a Protein complex structure. Full crystallographic information is available from OCA.

ReferenceReference

R6 hexameric insulin complexed with m-cresol or resorcinol., Smith GD, Ciszak E, Magrum LA, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1541-8. PMID:11092919 Page seeded by OCA on Fri May 2 15:33:19 2008

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OCA

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 [[Image:1ev6.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ev6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ev6 OCA], [http://www.ebi.ac.uk/pdbsum/1ev6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ev6 RCSB]</span>
-}}
 '''Structure of the monoclinic form of the M-cresol/insulin R6 hexamer'''
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 ==About this Structure==
-EV6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV6 OCA].
+EV6 is a [[Protein complex]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV6 OCA].
 ==Reference==
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 [[Category: Pangborn, W A.]]
 [[Category: Smith, G D.]]
-[[Category: r6 hexameric insulin]]
+[[Category: R6 hexameric insulin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:33:19 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:10:10 2008''