Isopeptide bond: Difference between revisions
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*The capsid of the HK97 bacteriophage contains isopeptide bonds that cross-link the protein [[chains]] into a protein '''chain mail''' <ref>PMID: 22297521</ref><ref>PMID: 10572124</ref><ref name="wikoff2000">PMID: 19091865</ref>. However, these do not occur in the [[asymmetric unit]] of [[1ohg]]<ref>PMID: 11000116</ref>. They can be visualized in the [[biological unit]], which is the full virus capsid, but that is technically challenging. | *The capsid of the HK97 bacteriophage contains isopeptide bonds that cross-link the protein [[chains]] into a protein '''chain mail''' <ref>PMID: 22297521</ref><ref>PMID: 10572124</ref><ref name="wikoff2000">PMID: 19091865</ref>. However, these do not occur in the [[asymmetric unit]] of [[1ohg]]<ref>PMID: 11000116</ref>. They can be visualized in the [[biological unit]], which is the full virus capsid, but that is technically challenging. | ||
* [[7cap]] is a ubiquitin homotrimer. [[3alb|3ALB]] is a ubiquitin homotetramer. In both cases, the <b>C-terminal</b> Gly76 is isopeptide linked to Lys48 in each '''inter-chain''' interface<ref name="hiranyakorn" />. | * [[7cap]] is a ubiquitin homotrimer. [[3alb|3ALB]] is a ubiquitin homotetramer. In both cases, the <b>C-terminal</b> Gly76 is isopeptide linked to Lys48 in each '''inter-chain''' interface<ref name="hiranyakorn" />. | ||
* [[4mli]] contains the SpyTag peptide (which can be linked to a protein of interest) forming an isopeptide bond between SpyTag peptide Lys31 and SpyCatcher protein Asp117<ref>PMID: 24161952</ref>. | * [[4mli]] contains the '''SpyTag peptide''' (which can be linked to a protein of interest) forming an isopeptide bond between SpyTag peptide Lys31 and SpyCatcher protein Asp117<ref>PMID: 24161952</ref>. | ||
* [[3htl]] is a pilus monomer with spontaneously-formed <b>intra-chain</b> isopeptide bonds Lys199-Asn321 and Lys363-Asn482<ref>PMID: 19805181</ref>. (Pilin chains form enzymatically, linked by the isopeptide Lys169 sidechain bonded to the carboxy terminus Thr494. Those inter-chain isopeptide bonds are not present in the monomer structure.) | * [[3htl]] is a pilus monomer with spontaneously-formed <b>intra-chain</b> isopeptide bonds Lys199-Asn321 and Lys363-Asn482<ref>PMID: 19805181</ref>. (Pilin chains form enzymatically, linked by the isopeptide Lys169 sidechain bonded to the carboxy terminus Thr494. Those inter-chain isopeptide bonds are not present in the monomer structure.) | ||
* [[6vzx]]: triple helix formed from collagen-mimetic peptides, stabilized by Lys11-Glu13 inter-chain isopeptide bonds. | * [[6vzx]]: triple helix formed from collagen-mimetic peptides, stabilized by Lys11-Glu13 inter-chain isopeptide bonds. | ||