1emy: Difference between revisions

Revision as of 15:17, 2 May 2008

CRYSTAL STRUCTURE OF ASIAN ELEPHANT (ELEPHAS MAXIMUS) CYANO-MET MYOGLOBIN AT 1.78 ANGSTROMS RESOLUTION. PHE 29 (B10) ACCOUNTS FOR ITS UNUSUAL LIGAND BINDING PROPERTIES

OverviewOverview

The crystal structure of Asian elephant cyano-metmyoglobin which has a glutamine instead of the usual distal site histidine has been determined to high resolution. In addition to this replacement, the substitution of a conserved leucine residue in position 29(B10) at the distal side by a phenylalanine was unambiguously identified based on the available electron density. The suspicion, that there were errors in the original sequence which has caused some confusion, is thus confirmed. Comparison with other myoglobin structures in various ligated forms reveals an essentially unchanged tertiary structure in elephant myoglobin despite the two amino acid substitutions in the heme pocket. Our current structural model shows that the N epsilon 2 atom of Gln64(E7) has moved with respect to the corresponding nitrogen position of His64(E7) in the CO complex of sperm whale myoglobin. The newly assigned residue Phe29(B10) penetrates into the distal side of the heme pocket approaching the ligand within van der Waals distance and causing a much more crowded heme pocket compared to other myoglobins. Kinetic properties of Asian elephant myoglobin, wild type, and recombinant sperm whale myoglobins are discussed in relation to the structural consequences of the two amino acid substitutions H64Q and L29F.

About this StructureAbout this Structure

1EMY is a Single protein structure of sequence from Elephas maximus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of Asian elephant (Elephas maximus) cyano-metmyoglobin at 1.78-A resolution. Phe29(B10) accounts for its unusual ligand binding properties., Bisig DA, Di Iorio EE, Diederichs K, Winterhalter KH, Piontek K, J Biol Chem. 1995 Sep 1;270(35):20754-62. PMID:7657658 Page seeded by OCA on Fri May 2 15:17:51 2008

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OCA

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 [[Image:1emy.gif|left|200px]]
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 '''CRYSTAL STRUCTURE OF ASIAN ELEPHANT (ELEPHAS MAXIMUS) CYANO-MET MYOGLOBIN AT 1.78 ANGSTROMS RESOLUTION. PHE 29 (B10) ACCOUNTS FOR ITS UNUSUAL LIGAND BINDING PROPERTIES'''
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 [[Category: Bisig, D A.]]
 [[Category: Piontek, K.]]
-[[Category: globin fold]]
+[[Category: Globin fold]]
-[[Category: heme protein]]
+[[Category: Heme protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:17:51 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:05:44 2008''