1em7: Difference between revisions

Revision as of 15:16, 2 May 2008

HELIX VARIANT OF THE B1 DOMAIN FROM STREPTOCOCCAL PROTEIN G

OverviewOverview

Six helix surface positions of protein G (Gbeta1) were redesigned using a computational protein design algorithm, resulting in the five fold mutant Gbeta1m2. Gbeta1m2 is well folded with a circular dichroism spectrum nearly identical to that of Gbeta1, and a melting temperature of 91 degrees C, approximately 6 degrees C higher than that of Gbeta1. The crystal structure of Gbeta1m2 was solved to 2.0 A resolution by molecular replacement. The absence of hydrogen bond or salt bridge interactions between the designed residues in Gbeta1m2 suggests that the increased stability of Gbeta1m2 is due to increased helix propensity and more favorable helix dipole interactions.

About this StructureAbout this Structure

1EM7 is a Single protein structure of sequence from Streptococcus sp.. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a protein G helix variant suggests the importance of helix propensity and helix dipole interactions in protein design., Strop P, Marinescu AM, Mayo SL, Protein Sci. 2000 Jul;9(7):1391-4. PMID:10933505 Page seeded by OCA on Fri May 2 15:16:08 2008

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OCA

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 [[Image:1em7.jpg|left|200px]]
- {{Structure
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-|RELATEDENTRY=[[1gb4|1GB4]], [[1pga|1PGA]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1em7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1em7 OCA], [http://www.ebi.ac.uk/pdbsum/1em7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1em7 RCSB]</span>
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 '''HELIX VARIANT OF THE B1 DOMAIN FROM STREPTOCOCCAL PROTEIN G'''
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 [[Category: Mayo, S L.]]
 [[Category: Strop, P.]]
-[[Category: helix dipole interaction]]
+[[Category: Helix dipole interaction]]
-[[Category: helix propensity]]
+[[Category: Helix propensity]]
-[[Category: protein design]]
+[[Category: Protein design]]
-[[Category: protein g]]
+[[Category: Protein g]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:16:08 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:05:20 2008''