1ekj: Difference between revisions

Revision as of 15:12, 2 May 2008

THE X-RAY CRYSTALLOGRAPHIC STRUCTURE OF BETA CARBONIC ANHYDRASE FROM THE C3 DICOT PISUM SATIVUM

OverviewOverview

We have determined the structure of the beta-carbonic anhydrase from the dicotyledonous plant Pisum sativum at 1.93 A resolution, using a combination of multiple anomalous scattering off the active site zinc ion and non-crystallographic symmetry averaging. The mol- ecule assembles as an octamer with a novel dimer of dimers of dimers arrangement. Two distinct patterns of conservation of active site residues are observed, implying two potentially mechanistically distinct classes of beta-carbonic anhydrases. The active site is located at the interface between two monomers, with Cys160, His220 and Cys223 binding the catalytic zinc ion and residues Asp162 (oriented by Arg164), Gly224, Gln151, Val184, Phe179 and Tyr205 interacting with the substrate analogue, acetic acid. The substrate binding groups have a one to one correspondence with the functional groups in the alpha-carbonic anhydrase active site, with the corresponding residues being closely superimposable by a mirror plane. Therefore, despite differing folds, alpha- and beta-carbonic anhydrase have converged upon a very similar active site design and are likely to share a common mechanism.

About this StructureAbout this Structure

1EKJ is a Single protein structure of sequence from Pisum sativum. Full crystallographic information is available from OCA.

ReferenceReference

The active site architecture of Pisum sativum beta-carbonic anhydrase is a mirror image of that of alpha-carbonic anhydrases., Kimber MS, Pai EF, EMBO J. 2000 Apr 3;19(7):1407-18. PMID:10747009 Page seeded by OCA on Fri May 2 15:12:57 2008

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OCA

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 [[Image:1ekj.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ekj |SIZE=350|CAPTION= <scene name='initialview01'>1ekj</scene>, resolution 1.93&Aring;
+The line below this paragraph, containing "STRUCTURE_1ekj", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1ekj|  PDB=1ekj  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ekj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ekj OCA], [http://www.ebi.ac.uk/pdbsum/1ekj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ekj RCSB]</span>
-}}
 '''THE X-RAY CRYSTALLOGRAPHIC STRUCTURE OF BETA CARBONIC ANHYDRASE FROM THE C3 DICOT PISUM SATIVUM'''
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 [[Category: Kimber, M S.]]
 [[Category: Pai, E F.]]
-[[Category: rossman fold domain]]
+[[Category: Rossman fold domain]]
-[[Category: strand exchange]]
+[[Category: Strand exchange]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:12:57 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:04:24 2008''