1ek0: Difference between revisions

Revision as of 15:11, 2 May 2008

GPPNHP-BOUND YPT51 AT 1.48 A RESOLUTION

OverviewOverview

Ypt/Rab proteins are membrane-associated small GTP-binding proteins which play a central role in the coordination, activation and regulation of vesicle-mediated transport in eukaryotic cells. We present the 1.5 A high-resolution crystal structure of Ypt51 in its active, GppNHp-bound conformation. Ypt51 is an important regulator involved in the endocytic membrane traffic of Saccharomyces cerevisiae. The structure reveals small but significant structural differences compared with H-Ras p21. The effector loop and the catalytic loop are well defined and stabilized by extensive hydrophobic interactions. The switch I and switch II regions form a well-defined epitope for hypothetical effector protein binding. Sequence comparisons between the different isoforms Ypt51, Ypt52 and Ypt53 provide the first insights into determinants for specific effector binding and for fine-tuning of the intrinsic GTP-hydrolysis rate.

About this StructureAbout this Structure

1EK0 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

High-resolution crystal structure of S. cerevisiae Ypt51(DeltaC15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis., Esters H, Alexandrov K, Constantinescu AT, Goody RS, Scheidig AJ, J Mol Biol. 2000 Apr 21;298(1):111-21. PMID:10756108 Page seeded by OCA on Fri May 2 15:11:51 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ek0.jpg|left|200px]]
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 '''GPPNHP-BOUND YPT51 AT 1.48 A RESOLUTION'''
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 [[Category: Esters, H.]]
 [[Category: Scheidig, A J.]]
-[[Category: endocytosis]]
+[[Category: Endocytosis]]
-[[Category: g protein]]
+[[Category: G protein]]
-[[Category: gtp hydrolysis]]
+[[Category: Gtp hydrolysis]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: vesicular traffic]]
+[[Category: Vesicular traffic]]
-[[Category: ypt/rab protein]]
+[[Category: Ypt/rab protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:11:51 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:04:09 2008''