Lysine-cysteine NOS bonds: Difference between revisions
Eric Martz (talk | contribs) |
Eric Martz (talk | contribs) |
||
| Line 19: | Line 19: | ||
Electron density for an unidentified atom appeared between the sidechain nitrogen of Lys8 and the sulfur of Cys38 for several crystals under nonreducing conditions, as well as in data from a low-dose non-synchrotron source, arguing against a radiation damage artifact. Competitive refinements indicated that the unidentified atom was oxygen, rather than carbon. | Electron density for an unidentified atom appeared between the sidechain nitrogen of Lys8 and the sulfur of Cys38 for several crystals under nonreducing conditions, as well as in data from a low-dose non-synchrotron source, arguing against a radiation damage artifact. Competitive refinements indicated that the unidentified atom was oxygen, rather than carbon. | ||
In crystals of reduced ( | In crystals of reduced (active) enzyme, the Lys8-O-Cys38 bridge was absent. However, electron density near the Cys38 sulfur was consistent with molecular oxygen O<sub>2</sub>. Molecular oxygen was absent in this position in the oxidized (active) enzyme. | ||
(to be continued, [[User:Eric Martz|Eric Martz]] 18:29, 26 May 2021 (UTC)) | (to be continued, [[User:Eric Martz|Eric Martz]] 18:29, 26 May 2021 (UTC)) | ||