1n1k: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
<StructureSection load='1n1k' size='340' side='right'caption='[[1n1k]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | <StructureSection load='1n1k' size='340' side='right'caption='[[1n1k]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1n1k]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N1K OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[1n1k]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N1K FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n1k OCA], [https://pdbe.org/1n1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n1k RCSB], [https://www.ebi.ac.uk/pdbsum/1n1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n1k ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
Revision as of 12:31, 5 May 2021
NMR Structure for d(CCGCGG)2NMR Structure for d(CCGCGG)2
Structural highlights
Publication Abstract from PubMedLong repeated stretches of d(CCG) and tri-nucleotide are crucial mutations that cause hereditary forms of mental retardation (fragile X-syndrome). Moreover, the alternating (CG) di-nucleotide is one of the candidates for Z-DNA conformation. Solution NMR structure of d(CCGCGG)(2) has been solved and is discussed. The determined NMR solution structure is a distorted highly bent B-DNA conformation with increased flexibility in both terminal residues. This conformation differs significantly from the Z-DNA tetramer structure reported for the same hexamer in the crystal state at similar ionic strength by Malinina and co-workers. Crystal structure of d(CCGCGG)(2) at high salt concentration includes a central alternating tetramer in Z-DNA conformation, while the initial cytosine swings out and forms a Watson-Crick base-pair with the terminal guanine of a symmetry-related molecule. In solution, NMR data for sugar ring puckering combined with restrained molecular dynamics simulations starting from a Z-DNA form show that terminal furanose residues could adopt the conformation required for aromatic bases swinging out. Therefore, tetramer formation could be considered possible once the hexanucleotide had previously adopted the Z-DNA form. This work gives some insight into correlations between anomalous crystal structures and their accessibility in the solution state. NMR study of hexanucleotide d(CCGCGG)2 containing two triplet repeats of fragile X syndrome.,Monleon D, Esteve V, Celda B Biochem Biophys Res Commun. 2003 Mar 28;303(1):81-90. PMID:12646170[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
| ||||||||||||||