6u9f

Structure of Francisella PdpA-VgrG Complex, LiddedStructure of Francisella PdpA-VgrG Complex, Lidded

6u9f, resolution 4.35Å Show:Asymmetric Unit Biological Assembly Structural highlights 6u9f is a 6 chain structure with sequence from Fratn. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: pdpA, FTN_1309 (FRATN), FTN_1312 (FRATN) Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Francisella bacteria rely on a phylogenetically distinct type VI secretion system (T6SS) to escape host phagosomes and cause the fatal disease tularemia, but the structural and molecular mechanisms involved are unknown. Here we report the atomic structure of the Francisella T6SS central spike complex, obtained by cryo-electron microscopy. Our structural and functional studies demonstrate that, unlike the single-protein spike composition of other T6SS subtypes, Francisella T6SS's central spike is formed by two proteins, PdpA and VgrG, akin to T4-bacteriophage gp27 and gp5, respectively, and that PdpA has unique characteristics, including a putative cargo within its cavity and an N-terminal helical lid. Structure-guided mutagenesis demonstrates that the PdpA N-terminal lid and C-terminal spike are essential to Francisella T6SS function. PdpA is thus both an adaptor, connecting VgrG to the tube, and a likely carrier of secreted cargo. These findings are important to understanding Francisella pathogenicity and designing therapeutics to combat tularemia. Atomic Structure of the Francisella T6SS Central Spike Reveals a Unique alpha-Helical Lid and a Putative Cargo.,Yang X, Clemens DL, Lee BY, Cui Y, Zhou ZH, Horwitz MA Structure. 2019 Dec 3;27(12):1811-1819.e6. doi: 10.1016/j.str.2019.10.007. Epub, 2019 Oct 31. PMID:31677891 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.