1ecl: Difference between revisions

Revision as of 14:56, 2 May 2008

AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1).

OverviewOverview

The three-dimensional structure of the 67K amino-terminal fragment of Escherichia coli DNA topoisomerase I has been determined to 2.2 A resolution. The polypeptide folds in an unusual way to give four distinct domains enclosing a hole large enough to accommodate a double-stranded DNA. The active-site tyrosyl residue, which is involved in the transient breakage of a DNA strand and the formation of a covalent enzyme-DNA intermediate, is present at the interface of two domains. The structure suggests a plausible mechanism by which E. coli DNA topoisomerase I and other members of the same DNA topoisomerase subfamily could catalyse the passage of one DNA strand through a transient break in another strand.

About this StructureAbout this Structure

1ECL is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Three-dimensional structure of the 67K N-terminal fragment of E. coli DNA topoisomerase I., Lima CD, Wang JC, Mondragon A, Nature. 1994 Jan 13;367(6459):138-46. PMID:8114910 Page seeded by OCA on Fri May 2 14:56:13 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1ecl.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ecl |SIZE=350|CAPTION= <scene name='initialview01'>1ecl</scene>, resolution 1.9&Aring;
+The line below this paragraph, containing "STRUCTURE_1ecl", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= TOPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_1ecl|  PDB=1ecl  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ecl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ecl OCA], [http://www.ebi.ac.uk/pdbsum/1ecl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ecl RCSB]</span>
-}}
 '''AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1).'''
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 [[Category: Mondragon, A.]]
 [[Category: Wang, J C.]]
-[[Category: bacterial type i]]
+[[Category: Bacterial type i]]
-[[Category: dna cleavage]]
+[[Category: Dna cleavage]]
-[[Category: strand passage]]
+[[Category: Strand passage]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:56:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:59:55 2008''