Sandbox GGC15: Difference between revisions
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'''The active site of Topo 1 is catalytic and it is the location where the nicking or cutting occurs<ref name="Redinbo" />. The nicking occurs from the trans-esterification of Tyr-723 at a DNA phophodiester bond forming a <scene name='78/781215/04_27_19_active_site_mech/1'>3'-phosphotyrosine covalent enzyme–DNA complex</scene> <ref name="Staker" />. After the DNA is relaxed, the covalent intermediate is reversed when the released 5'-OH of the broken strand reattacks the phosphotyrosine intermediate in a second transesterification reaction<ref name="Staker" />.''' | '''The active site of Topo 1 is catalytic and it is the location where the nicking or cutting occurs<ref name="Redinbo" />. The nicking occurs from the trans-esterification of Tyr-723 at a DNA phophodiester bond forming a <scene name='78/781215/04_27_19_active_site_mech/1'>3'-phosphotyrosine covalent enzyme–DNA complex</scene> <ref name="Staker" />. After the DNA is relaxed, the covalent intermediate is reversed when the released 5'-OH of the broken strand reattacks the phosphotyrosine intermediate in a second transesterification reaction<ref name="Staker" />.''' | ||
[[Image:Active Site | [[Image:Active Site 3PM.jpg]]<ref name ="Stewart">Stewart, L. (1998). A Model for the Mechanism of Human Topoisomerase I. Science, 279(5356), 1534–1541. https://doi.org/10.1126/science.279.5356.1534</ref> | ||
[[Image:DNA and Tyrosine.jpg]]<ref name="Stewart" /> | [[Image:DNA and Tyrosine.jpg]]<ref name="Stewart" /> | ||