1e8o: Difference between revisions

Revision as of 14:48, 2 May 2008

CORE OF THE ALU DOMAIN OF THE MAMMALIAN SRP

OverviewOverview

The Alu domain of the mammalian signal recognition particle (SRP) comprises the heterodimer of proteins SRP9 and SRP14 bound to the 5' and 3' terminal sequences of SRP RNA. It retards the ribosomal elongation of signal-peptide-containing proteins before their engagement with the translocation machinery in the endoplasmic reticulum. Here we report two crystal structures of the heterodimer SRP9/14 bound either to the 5' domain or to a construct containing both 5' and 3' domains. We present a model of the complete Alu domain that is consistent with extensive biochemical data. SRP9/14 binds strongly to the conserved core of the 5' domain, which forms a U-turn connecting two helical stacks. Reversible docking of the more weakly bound 3' domain might be functionally important in the mechanism of translational regulation. The Alu domain structure is probably conserved in other cytoplasmic ribonucleoprotein particles and retroposition intermediates containing SRP9/14-bound RNAs transcribed from Alu repeats or related elements in genomic DNA.

About this StructureAbout this Structure

1E8O is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure and assembly of the Alu domain of the mammalian signal recognition particle., Weichenrieder O, Wild K, Strub K, Cusack S, Nature. 2000 Nov 9;408(6809):167-73. PMID:11089964 Page seeded by OCA on Fri May 2 14:47:59 2008

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OCA

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 [[Image:1e8o.jpg|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e8o OCA], [http://www.ebi.ac.uk/pdbsum/1e8o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e8o RCSB]</span>
-}}
 '''CORE OF THE ALU DOMAIN OF THE MAMMALIAN SRP'''
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 [[Category: Weichenrieder, O.]]
 [[Category: Wild, K.]]
-[[Category: alu retroposition]]
+[[Category: Alu retroposition]]
-[[Category: alu ribonucleoprotein particle]]
+[[Category: Alu ribonucleoprotein particle]]
-[[Category: alu rnp assembly and transport]]
+[[Category: Alu rnp assembly and transport]]
-[[Category: protein recognition of an rna u-turn]]
+[[Category: Protein recognition of an rna u-turn]]
-[[Category: translational control]]
+[[Category: Translational control]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:47:59 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:57:31 2008''