1e86: Difference between revisions

Revision as of 14:47, 2 May 2008

CYTOCHROME C' FROM ALCALIGENES XYLOSOXIDANS-REDUCED STRUCTURE WITH CO BOUND TO DISTAL SIDE OF HEME

OverviewOverview

Microbial cytochromes c' contain a 5-coordinate His-ligated heme that forms stable adducts with nitric oxide (NO) and carbon monoxide (CO), but not with dioxygen. We report the 1.95 and 1.35 A resolution crystal structures of the CO- and NO-bound forms of the reduced protein from Alcaligenes xylosoxidans. NO disrupts the His-Fe bond and binds in a novel mode to the proximal face of the heme, giving a 5-coordinate species. In contrast, CO binds 6-coordinate on the distal side. A second CO molecule, not bound to the heme, is located in the proximal pocket. Since the unusual spectroscopic properties of cytochromes c' are shared by soluble guanylate cyclase (sGC), our findings have potential implications for the activation of sGC induced by the binding of NO or CO to the heme domain.

About this StructureAbout this Structure

1E86 is a Single protein structure of sequence from Achromobacter xylosoxidans. Full crystallographic information is available from OCA.

ReferenceReference

Unprecedented proximal binding of nitric oxide to heme: implications for guanylate cyclase., Lawson DM, Stevenson CE, Andrew CR, Eady RR, EMBO J. 2000 Nov 1;19(21):5661-71. PMID:11060017 Page seeded by OCA on Fri May 2 14:47:00 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1e86.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1e86 |SIZE=350|CAPTION= <scene name='initialview01'>1e86</scene>, resolution 1.95&Aring;
+The line below this paragraph, containing "STRUCTURE_1e86", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=CO1:Cmo+Binding+Site+For+Chain+A'>CO1</scene>, <scene name='pdbsite=CO2:Cmo+Binding+Site+For+Chain+A'>CO2</scene> and <scene name='pdbsite=HEC:Hec+Binding+Site+For+Chain+A'>HEC</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1e86|  PDB=1e86  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e86 OCA], [http://www.ebi.ac.uk/pdbsum/1e86 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e86 RCSB]</span>
-}}
 '''CYTOCHROME C' FROM ALCALIGENES XYLOSOXIDANS-REDUCED STRUCTURE WITH CO BOUND TO DISTAL SIDE OF HEME'''
@@ Line 30: / Line 27: @@
 [[Category: Stevenson, C E.M.]]
 [[Category: 4-helix bundle]]
-[[Category: carbon monoxide]]
+[[Category: Carbon monoxide]]
-[[Category: cytochrome]]
+[[Category: Cytochrome]]
-[[Category: heme]]
+[[Category: Heme]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:47:00 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:57:16 2008''