1e7p: Difference between revisions

Revision as of 14:46, 2 May 2008

QUINOL:FUMARATE REDUCTASE FROM WOLINELLA SUCCINOGENES

OverviewOverview

Quinol:fumarate reductase (QFR) is a membrane protein complex that couples the reduction of fumarate to succinate to the oxidation of quinol to quinone. Previously, the crystal structure of QFR from Wolinella succinogenes was determined based on two different crystal forms, and the site of fumarate binding in the flavoprotein subunit A of the enzyme was located between the FAD-binding domain and the capping domain [Lancaster, C.R.D., Kroger, A., Auer, M., & Michel, H. (1999) Nature 402, 377--385]. Here we describe the structure of W. succinogenes QFR based on a third crystal form and refined at 3.1 A resolution. Compared with the previous crystal forms, the capping domain is rotated in this structure by approximately 14 degrees relative to the FAD-binding domain. As a consequence, the topology of the dicarboxylate binding site is much more similar to those of membrane-bound and soluble fumarate reductase enzymes from other organisms than to that found in the previous crystal forms of W. succinogenes QFR. This and the effects of the replacement of Arg A301 by Glu or Lys by site-directed mutagenesis strongly support a common mechanism for fumarate reduction in this superfamily of enzymes.

About this StructureAbout this Structure

1E7P is a Protein complex structure of sequences from Wolinella succinogenes. Full crystallographic information is available from OCA.

ReferenceReference

A third crystal form of Wolinella succinogenes quinol:fumarate reductase reveals domain closure at the site of fumarate reduction., Lancaster CR, Gross R, Simon J, Eur J Biochem. 2001 Mar;268(6):1820-7. PMID:11248702 Page seeded by OCA on Fri May 2 14:46:03 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1e7p.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1e7p |SIZE=350|CAPTION= <scene name='initialview01'>1e7p</scene>, resolution 3.10&Aring;
+The line below this paragraph, containing "STRUCTURE_1e7p", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=FA1:Fad+Covalent+Bond+To+Protein+For+Chain+A'>FA1</scene>, <scene name='pdbsite=FA2:Fad+Covalent+Bond+To+Protein+For+Chain+D'>FA2</scene>, <scene name='pdbsite=FA3:Fad+Covalent+Bond+To+Protein+For+Chain+G'>FA3</scene>, <scene name='pdbsite=FA4:Fad+Covalent+Bond+To+Protein+For+Chain+K'>FA4</scene>, <scene name='pdbsite=FS1:Fe4s4+Fe+Sulphur+Centre+Ligands+For+Chain+B'>FS1</scene>, <scene name='pdbsite=FS2:Fe4s4+Fe+Sulphur+Centre+Ligands+For+Chain+E'>FS2</scene>, <scene name='pdbsite=FS3:Fe2s2+Fe+Sulphur+Centre+Ligands+For+Chain+B'>FS3</scene>, <scene name='pdbsite=FS4:Fe2s2+Fe+Sulphur+Centre+Ligands+For+Chain+E'>FS4</scene>, <scene name='pdbsite=FS5:Fe3s4+Fe+Sulphur+Centre+Ligands+For+Chain+B'>FS5</scene>, <scene name='pdbsite=FS6:Fe3s4+Fe+Sulphur+Centre+Ligands+For+Chain+E'>FS6</scene>, <scene name='pdbsite=FS7:Fe4s4+Fe+Sulphur+Centre+Ligands+For+Chain+G'>FS7</scene>, <scene name='pdbsite=FS8:Fe4s4+Fe+Sulphur+Centre+Ligands+For+Chain+K'>FS8</scene>, <scene name='pdbsite=FS9:Fe2s2+Fe+Sulphur+Centre+Ligands+For+Chain+G'>FS9</scene>, <scene name='pdbsite=FSA:Fe2s2+Fe+Sulphur+Centre+Ligands+For+Chain+K'>FSA</scene>, <scene name='pdbsite=FSB:Fe3s4+Fe+Sulphur+Centre+Ligands+For+Chain+G'>FSB</scene>, <scene name='pdbsite=FSC:Fe3s4+Fe+Sulphur+Centre+Ligands+For+Chain+K'>FSC</scene>, <scene name='pdbsite=HE1:Haem+Axial+Ligands+For+Chain+C'>HE1</scene>, <scene name='pdbsite=HE2:Haem+Axial+Ligands+For+Chain+F'>HE2</scene>, <scene name='pdbsite=HE3:Haem+Axial+Ligands+For+Chain+I'>HE3</scene> and <scene name='pdbsite=HE4:Haem+Axial+Ligands+For+Chain+L'>HE4</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Succinate_dehydrogenase Succinate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.1 1.3.99.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1e7p|  PDB=1e7p  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7p OCA], [http://www.ebi.ac.uk/pdbsum/1e7p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e7p RCSB]</span>
-}}
 '''QUINOL:FUMARATE REDUCTASE FROM WOLINELLA SUCCINOGENES'''
@@ Line 27: / Line 24: @@
 [[Category: Wolinella succinogenes]]
 [[Category: Lancaster, C R.D.]]
-[[Category: citric acid cycle]]
+[[Category: Citric acid cycle]]
-[[Category: dihaem cytochrome b]]
+[[Category: Dihaem cytochrome b]]
-[[Category: flavoprotein]]
+[[Category: Flavoprotein]]
-[[Category: fumarate reductase]]
+[[Category: Fumarate reductase]]
-[[Category: iron-sulphur protein]]
+[[Category: Iron-sulphur protein]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
-[[Category: respiratory chain]]
+[[Category: Respiratory chain]]
-[[Category: succinate dehydrogenase]]
+[[Category: Succinate dehydrogenase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:46:03 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:56:55 2008''