1aby: Difference between revisions
New page: left|200px<br /> <applet load="1aby" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aby, resolution 2.6Å" /> '''CYANOMET RHB1.1 (REC... |
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==Overview== | ==Overview== | ||
The need to develop a blood substitute is now urgent because of the, increasing concern over blood-transmitted viral and bacterial pathogens., Cell-free haemoglobin solutions and human haemoglobin synthesized in, Escherichia coli and Saccharomyces cerevisiae have been investigated as, potential oxygen-carrying substitutes for red blood cells. But these, haemoglobins cannot be used as a blood substitute because (1) the oxygen, affinity in the absence of 2,3-bisphosphoglycerate is too high to allow, unloading of enough oxygen in the tissues, and (2) they dissociate into, alpha beta dimers that are cleared rapidly by renal filtration, which can, result in long-term kidney damage. We have produced a human haemoglobin, using an expression vector containing one gene encoding a mutant, beta-globin with decreased oxygen affinity and one duplicated, tandemly, fused alpha-globin gene. Fusion of the two alpha-globin subunits increases, the half-life of this haemoglobin molecule in vivo by preventing its, dissociation into alpha beta dimers and therefore also eliminates renal, toxicity. | The need to develop a blood substitute is now urgent because of the, increasing concern over blood-transmitted viral and bacterial pathogens., Cell-free haemoglobin solutions and human haemoglobin synthesized in, Escherichia coli and Saccharomyces cerevisiae have been investigated as, potential oxygen-carrying substitutes for red blood cells. But these, haemoglobins cannot be used as a blood substitute because (1) the oxygen, affinity in the absence of 2,3-bisphosphoglycerate is too high to allow, unloading of enough oxygen in the tissues, and (2) they dissociate into, alpha beta dimers that are cleared rapidly by renal filtration, which can, result in long-term kidney damage. We have produced a human haemoglobin, using an expression vector containing one gene encoding a mutant, beta-globin with decreased oxygen affinity and one duplicated, tandemly, fused alpha-globin gene. Fusion of the two alpha-globin subunits increases, the half-life of this haemoglobin molecule in vivo by preventing its, dissociation into alpha beta dimers and therefore also eliminates renal, toxicity. | ||
==Disease== | |||
Known diseases associated with this structure: Erythremias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Erythremias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Erythrocytosis OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], HPFH, deletion type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Heinz body anemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Heinz body anemias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Heinz body anemias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Hemoglobin H disease OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Hypochromic microcytic anemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Methemoglobinemias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Methemoglobinemias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Sickle cell anemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Thalassemia, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Thalassemia-beta, dominant inclusion-body OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Thalassemias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Thalassemias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]] | |||
==About this Structure== | ==About this Structure== | ||
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[[Category: respiratory protein]] | [[Category: respiratory protein]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 15:57:38 2007'' | ||
