1e3m: Difference between revisions

Revision as of 14:37, 2 May 2008

THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:T MISMATCH

OverviewOverview

DNA mismatch repair ensures genomic integrity on DNA replication. Recognition of a DNA mismatch by a dimeric MutS protein initiates a cascade of reactions and results in repair of the newly synthesized strand; however, details of the molecular mechanism remain controversial. Here we present the crystal structure at 2.2 A of MutS from Escherichia coli bound to a G x T mismatch. The two MutS monomers have different conformations and form a heterodimer at the structural level. Only one monomer recognizes the mismatch specifically and has ADP bound. Mismatch recognition occurs by extensive minor groove interactions causing unusual base pairing and kinking of the DNA. Nonspecific major groove DNA-binding domains from both monomers embrace the DNA in a clamp-like structure. The interleaved nucleotide-binding sites are located far from the DNA. Mutations in human MutS alpha (MSH2/MSH6) that lead to hereditary predisposition for cancer, such as hereditary non-polyposis colorectal cancer, can be mapped to this crystal structure.

About this StructureAbout this Structure

1E3M is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of DNA mismatch repair protein MutS binding to a G x T mismatch., Lamers MH, Perrakis A, Enzlin JH, Winterwerp HH, de Wind N, Sixma TK, Nature. 2000 Oct 12;407(6805):711-7. PMID:11048711 Page seeded by OCA on Fri May 2 14:37:13 2008

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OCA

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 [[Image:1e3m.gif|left|200px]]
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-|GENE= MUTS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3m OCA], [http://www.ebi.ac.uk/pdbsum/1e3m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e3m RCSB]</span>
-}}
 '''THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:T MISMATCH'''
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 [[Category: Wind, N De.]]
 [[Category: Winterwerp, H H.K.]]
-[[Category: dna binding]]
+[[Category: Dna binding]]
-[[Category: mismatch recognition]]
+[[Category: Mismatch recognition]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:37:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:54:25 2008''