1e2x: Difference between revisions
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'''FADR, FATTY ACID RESPONSIVE TRANSCRIPTION FACTOR FROM E. COLI''' | '''FADR, FATTY ACID RESPONSIVE TRANSCRIPTION FACTOR FROM E. COLI''' | ||
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[[Category: Knudsen, J.]] | [[Category: Knudsen, J.]] | ||
[[Category: Wierenga, R K.]] | [[Category: Wierenga, R K.]] | ||
[[Category: | [[Category: Transcriptional regulation]] | ||
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Revision as of 14:35, 2 May 2008
FADR, FATTY ACID RESPONSIVE TRANSCRIPTION FACTOR FROM E. COLI
OverviewOverview
FadR is a dimeric acyl coenzyme A (acyl CoA)-binding protein and transcription factor that regulates the expression of genes encoding fatty acid biosynthetic and degrading enzymes in Escherichia coli. Here, the 2.0 A crystal structure of full-length FadR is described, determined using multi-wavelength anomalous dispersion. The structure reveals a dimer and a two-domain fold, with DNA-binding and acyl-CoA-binding sites located in an N-terminal and C-terminal domain, respectively. The N-terminal domain contains a winged helix-turn-helix prokaryotic DNA-binding fold. Comparison with known structures and analysis of mutagenesis data delineated the site of interaction with DNA. The C-terminal domain has a novel fold, consisting of a seven-helical bundle with a crossover topology. Careful analysis of the structure, together with mutational and biophysical data, revealed a putative hydrophobic acyl-CoA-binding site, buried in the core of the seven-helical bundle. This structure aids in understanding FadR function at a molecular level, provides the first structural scaffold for the large GntR family of transcription factors, which are keys in the control of metabolism in bacterial pathogens, and could thus be a possible target for novel chemotherapeutic agents.
About this StructureAbout this Structure
1E2X is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of FadR, a fatty acid-responsive transcription factor with a novel acyl coenzyme A-binding fold., van Aalten DM, DiRusso CC, Knudsen J, Wierenga RK, EMBO J. 2000 Oct 2;19(19):5167-77. PMID:11013219 Page seeded by OCA on Fri May 2 14:35:30 2008