1e0k: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1e0k.jpg|left|200px]] | [[Image:1e0k.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1e0k", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_1e0k| PDB=1e0k | SCENE= }} | |||
| | |||
}} | |||
'''GP4D HELICASE FROM PHAGE T7''' | '''GP4D HELICASE FROM PHAGE T7''' | ||
| Line 29: | Line 26: | ||
[[Category: Singleton, M R.]] | [[Category: Singleton, M R.]] | ||
[[Category: Wigley, D B.]] | [[Category: Wigley, D B.]] | ||
[[Category: | [[Category: Atpase]] | ||
[[Category: | [[Category: Dna replication]] | ||
[[Category: | [[Category: Helicase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:30:39 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 14:30, 2 May 2008
GP4D HELICASE FROM PHAGE T7
OverviewOverview
We have determined the crystal structure of an active, hexameric fragment of the gene 4 helicase from bacteriophage T7. The structure reveals how subunit contacts stabilize the hexamer. Deviation from expected six-fold symmetry of the hexamer indicates that the structure is of an intermediate on the catalytic pathway. The structural consequences of the asymmetry suggest a "binding change" mechanism to explain how cooperative binding and hydrolysis of nucleotides are coupled to conformational changes in the ring that most likely accompany duplex unwinding. The structure of a complex with a nonhydrolyzable ATP analog provides additional evidence for this hypothesis, with only four of the six possible nucleotide binding sites being occupied in this conformation of the hexamer. This model suggests a mechanism for DNA translocation.
About this StructureAbout this Structure
1E0K is a Single protein structure of sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides., Singleton MR, Sawaya MR, Ellenberger T, Wigley DB, Cell. 2000 Jun 9;101(6):589-600. PMID:10892646 Page seeded by OCA on Fri May 2 14:30:39 2008