1e0d: Difference between revisions

Revision as of 14:30, 2 May 2008

UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE

OverviewOverview

UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) is a cytoplasmic enzyme involved in the biosynthesis of peptidoglycan which catalyzes the addition of D-glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA). The crystal structure of MurD in the presence of its substrate UMA has been solved to 1.9 A resolution. Phase information was obtained from multiple anomalous dispersion using the K-shell edge of selenium in combination with multiple isomorphous replacement. The structure comprises three domains of topology each reminiscent of nucleotide-binding folds: the N- and C-terminal domains are consistent with the dinucleotide-binding fold called the Rossmann fold, and the central domain with the mononucleotide-binding fold also observed in the GTPase family. The structure reveals the binding site of the substrate UMA, and comparison with known NTP complexes allows the identification of residues interacting with ATP. The study describes the first structure of the UDP-N-acetylmuramoyl-peptide ligase family.

About this StructureAbout this Structure

1E0D is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli., Bertrand JA, Auger G, Fanchon E, Martin L, Blanot D, van Heijenoort J, Dideberg O, EMBO J. 1997 Jun 16;16(12):3416-25. PMID:9218784 Page seeded by OCA on Fri May 2 14:30:12 2008

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OCA

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 [[Image:1e0d.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1e0d |SIZE=350|CAPTION= <scene name='initialview01'>1e0d</scene>, resolution 2.40&Aring;
+The line below this paragraph, containing "STRUCTURE_1e0d", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylmuramoyl-L-alanine--D-glutamate_ligase UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.9 6.3.2.9] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= MURD GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e0d OCA], [http://www.ebi.ac.uk/pdbsum/1e0d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e0d RCSB]</span>
-}}
 '''UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE'''
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 [[Category: Dideberg, O.]]
 [[Category: Fanchon, E.]]
-[[Category: adp-forming enzyme]]
+[[Category: Adp-forming enzyme]]
-[[Category: ligase]]
+[[Category: Ligase]]
-[[Category: murd]]
+[[Category: Murd]]
-[[Category: peptidoglycan synthesis]]
+[[Category: Peptidoglycan synthesis]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:30:12 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:52:30 2008''