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'''ENDOGLUCANASE CEL6B FROM HUMICOLA INSOLENS''' | '''ENDOGLUCANASE CEL6B FROM HUMICOLA INSOLENS''' | ||
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[[Category: Schulein, M.]] | [[Category: Schulein, M.]] | ||
[[Category: Varrot, A.]] | [[Category: Varrot, A.]] | ||
[[Category: | [[Category: Cellulase]] | ||
[[Category: | [[Category: Cellulose degradation]] | ||
[[Category: | [[Category: Glycoside hydrolase family 6]] | ||
[[Category: | [[Category: Hydrolase]] | ||
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Revision as of 14:26, 2 May 2008
ENDOGLUCANASE CEL6B FROM HUMICOLA INSOLENS
OverviewOverview
Cellulases are traditionally classified as either endoglucanases or cellobiohydrolases on the basis of their respective catalytic activities on crystalline cellulose, which is generally hydrolysed more efficiently only by the cellobiohydrolases. On the basis of the Trichoderma reesei cellobiohydrolase II structure, it was proposed that the active-site tunnel of cellobiohydrolases permitted the processive hydrolysis of cellulose, whereas the corresponding endoglucanases would display open active-site clefts [Rouvinen, Bergfors, Teeri, Knowles and Jones (1990) Science 249, 380-386]. Glycoside hydrolase family 6 contains both cellobiohydrolases and endoglucanases. The structure of the catalytic core of the family 6 endoglucanase Cel6B from Humicola insolens has been solved by molecular replacement with the known T. reesei cellobiohydrolase II as the search model. Strangely, at the sequence level, this enzyme exhibits the highest sequence similarity to family 6 cellobiohydrolases and displays just one of the loop deletions traditionally associated with endoglucanases in this family. However, this enzyme shows no activity on crystalline substrates but a high activity on soluble substrates, which is typical of an endoglucanase. The three-dimensional structure reveals that the deletion of just a single loop of the active site, coupled with the resultant conformational change in a second 'cellobiohydrolase-specific' loop, peels open the active-site tunnel to reveal a substrate-binding groove.
About this StructureAbout this Structure
1DYS is a Single protein structure of sequence from Humicola insolens. Full crystallographic information is available from OCA.
ReferenceReference
Structure and function of Humicola insolens family 6 cellulases: structure of the endoglucanase, Cel6B, at 1.6 A resolution., Davies GJ, Brzozowski AM, Dauter M, Varrot A, Schulein M, Biochem J. 2000 May 15;348 Pt 1:201-7. PMID:10794732 Page seeded by OCA on Fri May 2 14:26:43 2008