6nb4

MERS-CoV S complex with human neutralizing LCA60 antibody Fab fragment (state 2)MERS-CoV S complex with human neutralizing LCA60 antibody Fab fragment (state 2)

6nb4, resolution 3.60Å Show:Asymmetric Unit Biological Assembly Structural highlights 6nb4 is a 5 chain structure with sequence from Human and Mers. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Related: 6nb3, 6nb5, 6nb6, 6nb7, 6nb8 Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Recent outbreaks of severe acute respiratory syndrome and Middle East respiratory syndrome, along with the threat of a future coronavirus-mediated pandemic, underscore the importance of finding ways to combat these viruses. The trimeric spike transmembrane glycoprotein S mediates entry into host cells and is the major target of neutralizing antibodies. To understand the humoral immune response elicited upon natural infections with coronaviruses, we structurally characterized the SARS-CoV and MERS-CoV S glycoproteins in complex with neutralizing antibodies isolated from human survivors. Although the two antibodies studied blocked attachment to the host cell receptor, only the anti-SARS-CoV S antibody triggered fusogenic conformational changes via receptor functional mimicry. These results provide a structural framework for understanding coronavirus neutralization by human antibodies and shed light on activation of coronavirus membrane fusion, which takes place through a receptor-driven ratcheting mechanism. Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion.,Walls AC, Xiong X, Park YJ, Tortorici MA, Snijder J, Quispe J, Cameroni E, Gopal R, Dai M, Lanzavecchia A, Zambon M, Rey FA, Corti D, Veesler D Cell. 2019 Jan 23. pii: S0092-8674(18)31642-8. doi: 10.1016/j.cell.2018.12.028. PMID:30712865 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.