1dyl: Difference between revisions

Revision as of 14:26, 2 May 2008

9 ANGSTROM RESOLUTION CRYO-EM RECONSTRUCTION STRUCTURE OF SEMLIKI FOREST VIRUS (SFV) AND FITTING OF THE CAPSID PROTEIN STRUCTURE IN THE EM DENSITY

OverviewOverview

Semliki Forest virus serves as a paradigm for membrane fusion and assembly. Our icosahedral reconstruction combined 5276 particle images from 48 cryo-electron micrographs and determined the virion structure to 9 A resolution. The improved resolution of this map reveals an N-terminal arm linking capsid subunits and defines the spike-capsid interaction sites. It illustrates the paired helical nature of the transmembrane segments and the elongated structures connecting them to the spike projecting domains. A 10 A diameter density in the fusion protein lines the cavity at the center of the spike. These clearly visible features combine with the variation in order between the layers to provide a framework for understanding the structural changes during the life cycle of an enveloped virus.

About this StructureAbout this Structure

1DYL is a Single protein structure of sequence from Semliki forest virus. Full crystallographic information is available from OCA.

ReferenceReference

Cryo-electron microscopy reveals the functional organization of an enveloped virus, Semliki Forest virus., Mancini EJ, Clarke M, Gowen BE, Rutten T, Fuller SD, Mol Cell. 2000 Feb;5(2):255-66. PMID:10882067 Page seeded by OCA on Fri May 2 14:26:22 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1dyl.gif|left|200px]]
- {{Structure
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 '''9 ANGSTROM RESOLUTION CRYO-EM RECONSTRUCTION STRUCTURE OF SEMLIKI FOREST VIRUS (SFV) AND FITTING OF THE CAPSID PROTEIN STRUCTURE IN THE EM DENSITY'''
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 [[Category: Mancini, E J.]]
 [[Category: Rutten, T.]]
-[[Category: alphavirus]]
+[[Category: Alphavirus]]
-[[Category: capsid protein]]
+[[Category: Capsid protein]]
-[[Category: cryo-em]]
+[[Category: Cryo-em]]
-[[Category: enveloped virus]]
+[[Category: Enveloped virus]]
-[[Category: icosahedral virus]]
+[[Category: Icosahedral virus]]
-[[Category: image reconstruction]]
+[[Category: Image reconstruction]]
-[[Category: sfv]]
+[[Category: Sfv]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:26:22 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:51:25 2008''