2gg6: Difference between revisions
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==CP4 EPSP synthase liganded with S3P== | ==CP4 EPSP synthase liganded with S3P== | ||
<StructureSection load='2gg6' size='340' side='right' caption='[[2gg6]], [[Resolution|resolution]] 1.64Å' scene=''> | <StructureSection load='2gg6' size='340' side='right'caption='[[2gg6]], [[Resolution|resolution]] 1.64Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2gg6]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2gg6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrsc Agrsc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GG6 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1g6t|1g6t]], [[1g6s|1g6s]], [[1q36|1q36]], [[1mi4|1mi4]], [[1rf5|1rf5]], [[1rf6|1rf6]], [[2gga|2gga]], [[2ggd|2ggd]], [[2gg4|2gg4]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g6t|1g6t]], [[1g6s|1g6s]], [[1q36|1q36]], [[1mi4|1mi4]], [[1rf5|1rf5]], [[1rf6|1rf6]], [[2gga|2gga]], [[2ggd|2ggd]], [[2gg4|2gg4]]</div></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aroA ([ | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aroA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=268951 AGRSC])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gg6 OCA], [https://pdbe.org/2gg6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gg6 RCSB], [https://www.ebi.ac.uk/pdbsum/2gg6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gg6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]] | [[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]] | ||
[[Category: Agrsc]] | [[Category: Agrsc]] | ||
[[Category: Large Structures]] | |||
[[Category: Funke, T]] | [[Category: Funke, T]] | ||
[[Category: Schonbrunn, E]] | [[Category: Schonbrunn, E]] | ||
Revision as of 22:01, 10 March 2021
CP4 EPSP synthase liganded with S3PCP4 EPSP synthase liganded with S3P
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe engineering of transgenic crops resistant to the broad-spectrum herbicide glyphosate has greatly improved agricultural efficiency worldwide. Glyphosate-based herbicides, such as Roundup, target the shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, the functionality of which is absolutely required for the survival of plants. Roundup Ready plants carry the gene coding for a glyphosate-insensitive form of this enzyme, obtained from Agrobacterium sp. strain CP4. Once incorporated into the plant genome, the gene product, CP4 EPSP synthase, confers crop resistance to glyphosate. Although widely used, the molecular basis for this glyphosate-resistance has remained obscure. We generated a synthetic gene coding for CP4 EPSP synthase and characterized the enzyme using kinetics and crystallography. The CP4 enzyme has unexpected kinetic and structural properties that render it unique among the known EPSP synthases. Glyphosate binds to the CP4 EPSP synthase in a condensed, noninhibitory conformation. Glyphosate sensitivity can be restored through a single-site mutation in the active site (Ala-100-Gly), allowing glyphosate to bind in its extended, inhibitory conformation. Molecular basis for the herbicide resistance of Roundup Ready crops.,Funke T, Han H, Healy-Fried ML, Fischer M, Schonbrunn E Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13010-5. Epub 2006 Aug 17. PMID:16916934[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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