7nfv: Difference between revisions
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==Structure of SARS-CoV-2 Papain-like protease PLpro== | ==Structure of SARS-CoV-2 Papain-like protease PLpro== | ||
<StructureSection load='7nfv' size='340' side='right'caption='[[7nfv]]' scene=''> | <StructureSection load='7nfv' size='340' side='right'caption='[[7nfv]], [[Resolution|resolution]] 1.42Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NFV FirstGlance]. <br> | <table><tr><td colspan='2'>[[7nfv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/2019-ncov 2019-ncov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NFV FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nfv OCA], [https://pdbe.org/7nfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nfv RCSB], [https://www.ebi.ac.uk/pdbsum/7nfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nfv ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nfv OCA], [https://pdbe.org/7nfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nfv RCSB], [https://www.ebi.ac.uk/pdbsum/7nfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nfv ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[https://www.uniprot.org/uniprot/R1A_SARS2 R1A_SARS2]] Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.[UniProtKB:P0C6X7] Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.[UniProtKB:P0C6X7] May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.[UniProtKB:P0C6X7] Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.[UniProtKB:P0C6X7] Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.[UniProtKB:P0C6X7] Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).[UniProtKB:P0C6X7] Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] May participate in viral replication by acting as a ssRNA-binding protein.[UniProtKB:P0C6X7] Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.[UniProtKB:P0C6X7] | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: 2019-ncov]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Andaleeb, H]] | ||
[[Category: | [[Category: Awel, S]] | ||
[[Category: | [[Category: Betzel, C]] | ||
[[Category: | [[Category: Brings, L]] | ||
[[Category: Brognaro H]] | [[Category: Brognaro, H]] | ||
[[Category: Ehrt C]] | [[Category: Ehrt, C]] | ||
[[Category: Ewert W]] | [[Category: Ewert, W]] | ||
[[Category: Fleckenstein H]] | [[Category: Falke, S]] | ||
[[Category: Galchenkova M]] | [[Category: Fleckenstein, H]] | ||
[[Category: Gelisio L]] | [[Category: Franca, B Alves]] | ||
[[Category: Gevorkov Y]] | [[Category: Galchenkova, M]] | ||
[[Category: Ginn H]] | [[Category: Gelisio, L]] | ||
[[Category: Groessler M]] | [[Category: Gevorkov, Y]] | ||
[[Category: Han H]] | [[Category: Ginn, H]] | ||
[[Category: Hinrichs W]] | [[Category: Groessler, M]] | ||
[[Category: Koua F]] | [[Category: Gunther, S]] | ||
[[Category: Lane | [[Category: Han, H]] | ||
[[Category: Li C]] | [[Category: Hinrichs, W]] | ||
[[Category: Lieske J]] | [[Category: Koua, F]] | ||
[[Category: Lorenzen K]] | [[Category: Lane, T J]] | ||
[[Category: Meents A]] | [[Category: Li, C]] | ||
[[Category: | [[Category: Lieske, J]] | ||
[[Category: | [[Category: Lorenzen, K]] | ||
[[Category: | [[Category: Meents, A]] | ||
[[Category: | [[Category: Perbandt, M]] | ||
[[Category: Perk, A]] | |||
[[Category: Saouane S]] | [[Category: Rarey, M]] | ||
[[Category: Schmidt C]] | [[Category: Reinke, P]] | ||
[[Category: Schubert R]] | [[Category: Saouane, S]] | ||
[[Category: Schwinzer M]] | [[Category: Schmidt, C]] | ||
[[Category: | [[Category: Schubert, R]] | ||
[[Category: | [[Category: Schwinzer, M]] | ||
[[Category: | [[Category: Sprenger, J]] | ||
[[Category: | [[Category: Srinivasan, V]] | ||
[[Category: | [[Category: Tolstikova, A]] | ||
[[Category: | [[Category: Trost, F]] | ||
[[Category: | [[Category: Ullah, N]] | ||
[[Category: | [[Category: Wang, M]] | ||
[[Category: | [[Category: Werner, N]] | ||
[[Category: Yefanov, O]] | |||
[[Category: Hydrolase]] | |||
[[Category: Papain-like protease sars-cov-2 hydrolase zinc binding protein]] | |||