1dug: Difference between revisions

Revision as of 14:17, 2 May 2008

STRUCTURE OF THE FIBRINOGEN G CHAIN INTEGRIN BINDING AND FACTOR XIIIA CROSSLINKING SITES OBTAINED THROUGH CARRIER PROTEIN DRIVEN CRYSTALLIZATION

OverviewOverview

The human fibrinogen gamma-chain C-terminal segment functions as the platelet integrin binding site as well as the Factor XIIIa cross-linking substrate and thus plays an important role in blood clot formation and stabilization. The three-dimensional structure of this segment has been determined using carrier protein driven crystallization. The C-terminal segment, gamma-(398-411), was attached to a linker sequence at the C-terminus of glutathione S-transferase and the structure of this fusion protein determined at 1.8 A resolution. Functional studies of the chimeric protein demonstrate that the fibrinogen sequence in the presence of the carrier protein retains its specific functions as ligand for platelet integrin alpha(IIb)beta3 (gpIIb/IIIa) and as a cross-linking substrate for Factor XIIIa. The structure obtained for the fibrinogen gamma-chain segment is not affected by crystal packing and can provide the missing links to the recently reported model of cross-linked fibrin.

About this StructureAbout this Structure

1DUG is a Single protein structure of sequence from Schistosoma japonicum. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the fibrinogen gamma-chain integrin binding and factor XIIIa cross-linking sites obtained through carrier protein driven crystallization., Ware S, Donahue JP, Hawiger J, Anderson WF, Protein Sci. 1999 Dec;8(12):2663-71. PMID:10631982 Page seeded by OCA on Fri May 2 14:17:21 2008

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OCA

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 [[Image:1dug.jpg|left|200px]]
- {{Structure
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-}}
 '''STRUCTURE OF THE FIBRINOGEN G CHAIN INTEGRIN BINDING AND FACTOR XIIIA CROSSLINKING SITES OBTAINED THROUGH CARRIER PROTEIN DRIVEN CRYSTALLIZATION'''
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 [[Category: Hawiger, J.]]
 [[Category: Ware, S.]]
-[[Category: carrier protein driven crystallization]]
+[[Category: Carrier protein driven crystallization]]
-[[Category: gamma chain integrin fragment]]
+[[Category: Gamma chain integrin fragment]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:17:21 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:48:57 2008''