Protein Hfq: Difference between revisions

The biological assembly of Protein Hfq from ''E. coli'' is <scene name='87/875687/Cv/2'>homohexamer</scene> (PDB code [[4ht9]]). The 3D structure of the complex between Hfq and <scene name='87/875687/Cv/5'>poly(A) RNA and poly(U) RNA</scene> shows the poly(A) binding at the distal face of Hfq and poly(U) binding at its proximal face. For the poly(A) A-R-N binding site the A site shows <scene name='87/875687/Cv/4'>hydrogen bonding to Gln and Lys</scene>.  The <scene name='87/875687/Cv/6'>R site shows stacking interactions as well as hydrogen bonding</scene>. The N site does not show any interaction. The poly(U) binding site of Hfq is a classical uridine binding pocket with both stacking and hydrogen bonding interactions<ref>PMID:23605038</ref>.