1dqy: Difference between revisions

Revision as of 14:10, 2 May 2008

CRYSTAL STRUCTURE OF ANTIGEN 85C FROM MYCOBACTERIUM TUBERCULOSIS WITH DIETHYL PHOSPHATE INHIBITOR

OverviewOverview

The antigen 85 (ag85) complex, composed of three proteins (ag85A, B and C), is a major protein component of the Mycobacterium tuberculosis cell wall. Each protein possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. The crystal structure of recombinant ag85C from M. tuberculosis, refined to a resolution of 1.5 A, reveals an alpha/beta-hydrolase polypeptide fold, and a catalytic triad formed by Ser 124, Glu 228 and His 260. ag85C complexed with a covalent inhibitor implicates residues Leu 40 and Met 125 as components of the oxyanion hole. A hydrophobic pocket and tunnel extending 21 A into the core of the protein indicates the location of a probable trehalose monomycolate binding site. Also, a large region of conserved surface residues among ag85A, B and C is a probable site for the interaction of ag85 proteins with human fibronectin.

About this StructureAbout this Structure

1DQY is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the secreted form of antigen 85C reveals potential targets for mycobacterial drugs and vaccines., Ronning DR, Klabunde T, Besra GS, Vissa VD, Belisle JT, Sacchettini JC, Nat Struct Biol. 2000 Feb;7(2):141-6. PMID:10655617 Page seeded by OCA on Fri May 2 14:10:26 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1dqy.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1dqy", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DEP:DIETHYL+PHOSPHONATE'>DEP</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>
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-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0627 COG0627], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam00756 Esterase]</span>
+{{STRUCTURE_1dqy|  PDB=1dqy  |  SCENE=  }}
-|RELATEDENTRY=[[1dqz|1DQZ]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dqy OCA], [http://www.ebi.ac.uk/pdbsum/1dqy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dqy RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF ANTIGEN 85C FROM MYCOBACTERIUM TUBERCULOSIS WITH DIETHYL PHOSPHATE INHIBITOR'''
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 [[Category: TBSGC, TB Structural Genomics Consortium.]]
 [[Category: 85c]]
-[[Category: antigen]]
+[[Category: Antigen]]
-[[Category: dpi]]
+[[Category: Dpi]]
-[[Category: fibronectin]]
+[[Category: Fibronectin]]
-[[Category: mycobacterium tuberculosis]]
+[[Category: Mycobacterium tuberculosis]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: tb structural genomics consortium]]
+[[Category: Tb structural genomics consortium]]
-[[Category: tbsgc]]
+[[Category: Tbsgc]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:10:26 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:47:02 2008''