1dpu: Difference between revisions

Revision as of 14:07, 2 May 2008

SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF HUMAN RPA32 COMPLEXED WITH UNG2(73-88)

OverviewOverview

Replication protein A (RPA), the nuclear ssDNA-binding protein in eukaryotes, is essential to DNA replication, recombination, and repair. We have shown that a globular domain at the C terminus of subunit RPA32 contains a specific surface that interacts in a similar manner with the DNA repair enzyme UNG2 and repair factors XPA and RAD52, each of which functions in a different repair pathway. NMR structures of the RPA32 domain, free and in complex with the minimal interaction domain of UNG2, were determined, defining a common structural basis for linking RPA to the nucleotide excision, base excision, and recombinational pathways of repairing damaged DNA. Our findings support a hand-off model for the assembly and coordination of different components of the DNA repair machinery.

About this StructureAbout this Structure

1DPU is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA., Mer G, Bochkarev A, Gupta R, Bochkareva E, Frappier L, Ingles CJ, Edwards AM, Chazin WJ, Cell. 2000 Oct 27;103(3):449-56. PMID:11081631 Page seeded by OCA on Fri May 2 14:07:49 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1dpu.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1dpu |SIZE=350|CAPTION= <scene name='initialview01'>1dpu</scene>
+The line below this paragraph, containing "STRUCTURE_1dpu", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1dpu|  PDB=1dpu  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dpu OCA], [http://www.ebi.ac.uk/pdbsum/1dpu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dpu RCSB]</span>
-}}
 '''SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF HUMAN RPA32 COMPLEXED WITH UNG2(73-88)'''
@@ Line 28: / Line 25: @@
 [[Category: Edwards, A M.]]
 [[Category: Mer, G.]]
-[[Category: dna repair]]
+[[Category: Dna repair]]
-[[Category: nmr]]
+[[Category: Nmr]]
-[[Category: protein-peptide complex]]
+[[Category: Protein-peptide complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:07:49 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:46:19 2008''