1dpf: Difference between revisions

Revision as of 14:07, 2 May 2008

CRYSTAL STRUCTURE OF A MG-FREE FORM OF RHOA COMPLEXED WITH GDP

OverviewOverview

Mg(2+) ions are essential for guanosine triphosphatase (GTPase) activity and play key roles in guanine nucleotide binding and preserving the structural integrity of GTP-binding proteins. We determined the crystal structure of a small GTPase RHOA complexed with GDP in the absence of Mg(2+) at 2.0-A resolution. Elimination of a Mg(2+) ion induces significant conformational changes in the switch I region that opens up the nucleotide-binding site. Similar structural changes have been observed in the switch regions of Ha-Ras bound to its guanine nucleotide exchange factor, Sos. This RHOA-GDP structure reveals an important regulatory role for Mg(2+) and suggests that guanine nucleotide exchange factor may utilize this feature of switch I to produce an open conformation in GDP/GTP exchange.

About this StructureAbout this Structure

1DPF is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

An open conformation of switch I revealed by the crystal structure of a Mg2+-free form of RHOA complexed with GDP. Implications for the GDP/GTP exchange mechanism., Shimizu T, Ihara K, Maesaki R, Kuroda S, Kaibuchi K, Hakoshima T, J Biol Chem. 2000 Jun 16;275(24):18311-7. PMID:10748207 Page seeded by OCA on Fri May 2 14:07:00 2008

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OCA

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 [[Image:1dpf.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dpf OCA], [http://www.ebi.ac.uk/pdbsum/1dpf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dpf RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF A MG-FREE FORM OF RHOA COMPLEXED WITH GDP'''
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 [[Category: Maesaki, R.]]
 [[Category: Shimizu, T.]]
-[[Category: protein rhoa-gdp complex]]
+[[Category: Protein rhoa-gdp complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:07:00 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:46:10 2008''