|
|
| Line 1: |
Line 1: |
|
| |
|
| ==Crystal structure of Saccharomyces cerevisiae Galactokinase in complex with galactose and Mg:AMPPNP== | | ==Crystal structure of Saccharomyces cerevisiae Galactokinase in complex with galactose and Mg:AMPPNP== |
| <StructureSection load='2aj4' size='340' side='right' caption='[[2aj4]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='2aj4' size='340' side='right'caption='[[2aj4]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[2aj4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AJ4 FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AJ4 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aj4 OCA], [https://pdbe.org/2aj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aj4 RCSB], [https://www.ebi.ac.uk/pdbsum/2aj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aj4 ProSAT]</span></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GAL1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
| |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactokinase Galactokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.6 2.7.1.6] </span></td></tr>
| |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aj4 OCA], [http://pdbe.org/2aj4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2aj4 RCSB], [http://www.ebi.ac.uk/pdbsum/2aj4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2aj4 ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| Line 19: |
Line 16: |
| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aj4 ConSurf]. | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aj4 ConSurf]. |
| <div style="clear:both"></div> | | <div style="clear:both"></div> |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| Gal1p of Saccharomyces cerevisiae is capable of performing two independent cellular functions. First, it is a key enzyme in the Leloir pathway for galactose metabolism where it catalyzes the conversion of alpha-d-galactose to galactose 1-phosphate. Second, it has the capacity to induce the transcription of the yeast GAL genes in response to the organism being challenged with galactose as the sole source of carbon. This latter function is normally performed by a highly related protein, Gal3p, but in its absence Gal1p can induce transcription, albeit inefficiently, both in vivo and in vitro. Here we report the x-ray structure of Gal1p in complex with alpha-d-galactose and Mg-adenosine 5'-(beta,gamma-imido)triphosphate (AMPPNP) determined to 2.4 Angstrom resolution. Overall, the enzyme displays a marked bilobal appearance with the active site being wedged between distinct N- and C-terminal domains. Despite being considerably larger than other galactokinases, Gal1p shares a similar molecular architecture with these enzymes as well as with other members of the GHMP superfamily. The extraordinary levels of similarity between Gal1p and Gal3p ( approximately 70% amino acid identity and approximately 90% similarity) have allowed a model for Gal3p to be constructed. By identifying the locations of mutations of Gal3p that result in altered transcriptional properties, we suggest potential models for Gal3p function and mechanisms for its interaction with the transcriptional inhibitor Gal80p. The GAL genetic switch has long been regarded as a paradigm for the control of gene expression in eukaryotes. Understanding the manner in which two of the proteins that function in transcriptional regulation interact with one another is an important step in determining the overall molecular mechanism of this switch.
| |
|
| |
|
| Molecular structure of Saccharomyces cerevisiae Gal1p, a bifunctional galactokinase and transcriptional inducer.,Thoden JB, Sellick CA, Timson DJ, Reece RJ, Holden HM J Biol Chem. 2005 Nov 4;280(44):36905-11. Epub 2005 Aug 22. PMID:16115868<ref>PMID:16115868</ref>
| | ==See Also== |
| | | *[[Galactokinase|Galactokinase]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 2aj4" style="background-color:#fffaf0;"></div>
| |
| == References == | |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Atcc 18824]] | | [[Category: Large Structures]] |
| [[Category: Galactokinase]]
| | [[Category: Holden HM]] |
| [[Category: Holden, H M]] | | [[Category: Reece RJ]] |
| [[Category: Reece, R J]] | | [[Category: Sellick CA]] |
| [[Category: Sellick, C A]] | | [[Category: Thoden JB]] |
| [[Category: Thoden, J B]] | | [[Category: Timson DJ]] |
| [[Category: Timson, D J]] | |
| [[Category: Galactosemia]]
| |
| [[Category: Transcription]]
| |
| [[Category: Transferase]]
| |
