1u3o: Difference between revisions
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==Solution structure of rat Kalirin N-terminal SH3 domain== | ==Solution structure of rat Kalirin N-terminal SH3 domain== | ||
<StructureSection load='1u3o' size='340' side='right' caption='[[1u3o]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | <StructureSection load='1u3o' size='340' side='right'caption='[[1u3o]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1u3o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U3O OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[1u3o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U3O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1U3O FirstGlance]. <br> | ||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kalirin, HAPIP, Duo ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kalirin, HAPIP, Duo ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1u3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u3o OCA], [http://pdbe.org/1u3o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u3o RCSB], [http://www.ebi.ac.uk/pdbsum/1u3o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u3o ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Buffalo rat]] | [[Category: Buffalo rat]] | ||
[[Category: Large Structures]] | |||
[[Category: Chakrabarti, K]] | [[Category: Chakrabarti, K]] | ||
[[Category: Eipper, B A]] | [[Category: Eipper, B A]] | ||
Revision as of 10:20, 30 December 2020
Solution structure of rat Kalirin N-terminal SH3 domainSolution structure of rat Kalirin N-terminal SH3 domain
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedRhoGEFs are central controllers of small G-proteins in cells and are regulated by several mechanisms. There are at least 22 human RhoGEFs that contain SH3 domains, raising the possibility that, like several other enzymes, SH3 domains control the enzymatic activity of guanine nucleotide exchange factor (GEF) domains through intra- and/or intermolecular interactions. The structure of the N-terminal SH3 domain of Kalirin was solved using NMR spectroscopy, and it folds much like other SH3 domains. However, NMR chemical shift mapping experiments showed that this Kalirin SH3 domain is unique, containing novel cooperative binding site(s) for intramolecular PXXP ligands. Intramolecular Kalirin SH3 domain/ligand interactions, as well as binding of the Kalirin SH3 domain to the adaptor protein Crk, inhibit the GEF activity of Kalirin. This study establishes a novel molecular mechanism whereby intramolecular and intermolecular Kalirin SH3 domain/ligand interactions modulate GEF activity, a regulatory mechanism that is likely used by other RhoGEF family members. Regulation of RhoGEF activity by intramolecular and intermolecular SH3 domain interactions.,Schiller MR, Chakrabarti K, King GF, Schiller NI, Eipper BA, Maciejewski MW J Biol Chem. 2006 Jul 7;281(27):18774-86. Epub 2006 Apr 27. PMID:16644733[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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