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==Crystal structure of human inorganic pyrophosphatase with metal ions== | ==Crystal structure of human inorganic pyrophosphatase with metal ions== | ||
<StructureSection load='7btn' size='340' side='right'caption='[[7btn]]' scene=''> | <StructureSection load='7btn' size='340' side='right'caption='[[7btn]], [[Resolution|resolution]] 2.38Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BTN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BTN FirstGlance]. <br> | <table><tr><td colspan='2'>[[7btn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BTN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BTN FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7btn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7btn OCA], [http://pdbe.org/7btn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7btn RCSB], [http://www.ebi.ac.uk/pdbsum/7btn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7btn ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PPA1, IOPPP, PP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7btn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7btn OCA], [http://pdbe.org/7btn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7btn RCSB], [http://www.ebi.ac.uk/pdbsum/7btn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7btn ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Inorganic pyrophosphatase (PPase) plays an essential role in energy conservation and provides energy for many biosynthetic pathways. Here, we present two three-dimensional structures of PPase from Homo sapiens (Hu-PPase) at 2.38 A and 3.40 A in different crystallization conditions. One of the Hu-PPase structures complex of two magnesium metal ions was determined to be a monomer (Hu-PPase-mono) here, while the other one to be a dimer-dimer (Hu-PPase-dd). In each asymmetric unit of Hu-PPase-mono, there are four alpha-helices and ten beta-strands and folds as a barrel structure, and the active site contains two magnesium ions. Like PPases from many species, we found that Hu-PPase was able to undergo self-assembly. To our surprise, disruption of the self-assembly of Hu-PPase did not influence its enzymatic activity or the ability to promote cell growth. Our work uncovered that different structure forms of Hu-PPase and found that the pyrophosphatase activity of Hu-PPase is independent of its self-assembly. | |||
Structural and biochemical characterization of inorganic pyrophosphatase from Homo sapiens.,Hu F, Huang Z, Zheng S, Wu Q, Chen Y, Lin H, Huang W, Li L Biochem Biophys Res Commun. 2020 Oct 6. pii: S0006-291X(20)31891-X. doi:, 10.1016/j.bbrc.2020.09.139. PMID:33036755<ref>PMID:33036755</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7btn" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Human]] | |||
[[Category: Inorganic diphosphatase]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Hu F]] | [[Category: Hu, F]] | ||
[[Category: Huang Z]] | [[Category: Huang, Z]] | ||
[[Category: Li L]] | [[Category: Li, L]] | ||
[[Category: Complex]] | |||
[[Category: Hydrolase]] | |||
[[Category: Monomer]] | |||