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==DHNA complex with 8-Amino-1,3-dimethyl-3,7-dihydropurine-2,6-dione==
==DHNA complex with 8-Amino-1,3-dimethyl-3,7-dihydropurine-2,6-dione==
<StructureSection load='1rs2' size='340' side='right' caption='[[1rs2]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
<StructureSection load='1rs2' size='340' side='right'caption='[[1rs2]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1rs2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RS2 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1rs2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RS2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RS2 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=209:8-AMINO-1,3-DIMETHYL-3,7-DIHYDROPURINE-2,6-DIONE'>209</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=209:8-AMINO-1,3-DIMETHYL-3,7-DIHYDROPURINE-2,6-DIONE'>209</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rr5|1rr5]], [[1rri|1rri]], [[1rrw|1rrw]], [[1rry|1rry]], [[1rs4|1rs4]], [[1rsd|1rsd]], [[1rsi|1rsi]]</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rr5|1rr5]], [[1rri|1rri]], [[1rrw|1rrw]], [[1rry|1rry]], [[1rs4|1rs4]], [[1rsd|1rsd]], [[1rsi|1rsi]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FOLB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FOLB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydroneopterin_aldolase Dihydroneopterin aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.25 4.1.2.25] </span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydroneopterin_aldolase Dihydroneopterin aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.25 4.1.2.25] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rs2 OCA], [http://pdbe.org/1rs2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rs2 RCSB], [http://www.ebi.ac.uk/pdbsum/1rs2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rs2 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1rs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rs2 OCA], [http://pdbe.org/1rs2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rs2 RCSB], [http://www.ebi.ac.uk/pdbsum/1rs2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rs2 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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==See Also==
==See Also==
*[[Aldolase|Aldolase]]
*[[Aldolase 3D structures|Aldolase 3D structures]]
*[[Chorismate synthase|Chorismate synthase]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Dihydroneopterin aldolase]]
[[Category: Dihydroneopterin aldolase]]
[[Category: Large Structures]]
[[Category: Betz, S F]]
[[Category: Betz, S F]]
[[Category: Beutel, B A]]
[[Category: Beutel, B A]]

Revision as of 16:32, 16 December 2020

DHNA complex with 8-Amino-1,3-dimethyl-3,7-dihydropurine-2,6-dioneDHNA complex with 8-Amino-1,3-dimethyl-3,7-dihydropurine-2,6-dione

Structural highlights

1rs2 is a 1 chain structure with sequence from "micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:FOLB ("Micrococcus aureus" (Rosenbach 1884) Zopf 1885)
Activity:Dihydroneopterin aldolase, with EC number 4.1.2.25
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FOLB_STAAU] Catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Potent inhibitors of 7,8-dihydroneopterin aldolase (DHNA; EC 4.1.2.25) have been discovered using CrystaLEAD X-ray crystallographic high-throughput screening followed by structure-directed optimization. Screening of a 10 000 compound random library provided several low affinity leads and their corresponding X-ray crystal structures bound to the enzyme. The presence of a common structural feature in each of the leads suggested a strategy for the construction of a directed library of approximately 1000 compounds that were screened for inhibitory activity in a traditional enzyme assay. Several lead compounds with IC(50) values of about 1 microM against DHNA were identified, and crystal structures of their enzyme-bound complexes were obtained by cocrystallization. Structure-directed optimization of one of the leads thus identified afforded potent inhibitors with submicromolar IC(50) values.

Discovery of potent inhibitors of dihydroneopterin aldolase using CrystaLEAD high-throughput X-ray crystallographic screening and structure-directed lead optimization.,Sanders WJ, Nienaber VL, Lerner CG, McCall JO, Merrick SM, Swanson SJ, Harlan JE, Stoll VS, Stamper GF, Betz SF, Condroski KR, Meadows RP, Severin JM, Walter KA, Magdalinos P, Jakob CG, Wagner R, Beutel BA J Med Chem. 2004 Mar 25;47(7):1709-18. PMID:15027862[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Sanders WJ, Nienaber VL, Lerner CG, McCall JO, Merrick SM, Swanson SJ, Harlan JE, Stoll VS, Stamper GF, Betz SF, Condroski KR, Meadows RP, Severin JM, Walter KA, Magdalinos P, Jakob CG, Wagner R, Beutel BA. Discovery of potent inhibitors of dihydroneopterin aldolase using CrystaLEAD high-throughput X-ray crystallographic screening and structure-directed lead optimization. J Med Chem. 2004 Mar 25;47(7):1709-18. PMID:15027862 doi:http://dx.doi.org/10.1021/jm030497y

1rs2, resolution 2.31Å

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