1kcf: Difference between revisions
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==Crystal Structure of the Yeast Mitochondrial Holliday Junction Resolvase, Ydc2== | ==Crystal Structure of the Yeast Mitochondrial Holliday Junction Resolvase, Ydc2== | ||
<StructureSection load='1kcf' size='340' side='right' caption='[[1kcf]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='1kcf' size='340' side='right'caption='[[1kcf]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1kcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KCF OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[1kcf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KCF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1KCF FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPAC25G10.02 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPAC25G10.02 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1kcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kcf OCA], [http://pdbe.org/1kcf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kcf RCSB], [http://www.ebi.ac.uk/pdbsum/1kcf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1kcf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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</div> | </div> | ||
<div class="pdbe-citations 1kcf" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1kcf" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Resolvase|Resolvase]] | |||
*[[Resolvase 3D structures|Resolvase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Cbs 356]] | [[Category: Cbs 356]] | ||
[[Category: Large Structures]] | |||
[[Category: Barrett, T E]] | [[Category: Barrett, T E]] | ||
[[Category: Ceschini, S]] | [[Category: Ceschini, S]] | ||
Revision as of 10:43, 25 November 2020
Crystal Structure of the Yeast Mitochondrial Holliday Junction Resolvase, Ydc2Crystal Structure of the Yeast Mitochondrial Holliday Junction Resolvase, Ydc2
Structural highlights
Function[CCE1_SCHPO] Capable of resolving Holliday junctions. Specific for 4-way junctions. Seems to be important for the maintenance of mitochondrial DNA. Cleaves fixed junctions at the point of strand exchange. Cleaves after 5'-CT-3' and 5'-TT-3' sequences.[1] [2] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedResolution of Holliday junctions into separate DNA duplexes requires enzymatic cleavage of an equivalent strand from each contributing duplex at or close to the point of strand exchange. Diverse Holliday junction-resolving enzymes have been identified in bacteria, bacteriophages, archaea and pox viruses, but the only eukaryotic examples identified so far are those from fungal mitochondria. We have now determined the crystal structure of Ydc2 (also known as SpCce1), a Holliday junction resolvase from the fission yeast Schizosaccharomyces pombe that is involved in the maintenance of mitochondrial DNA. This first structure of a eukaryotic Holliday junction resolvase confirms a distant evolutionary relationship to the bacterial RuvC family, but reveals structural features which are unique to the eukaryotic enzymes. Detailed analysis of the dimeric structure suggests mechanisms for junction isomerization and communication between the two active sites, and together with site-directed mutagenesis identifies residues involved in catalysis. Crystal structure of the fission yeast mitochondrial Holliday junction resolvase Ydc2.,Ceschini S, Keeley A, McAlister MS, Oram M, Phelan J, Pearl LH, Tsaneva IR, Barrett TE EMBO J. 2001 Dec 3;20(23):6601-11. PMID:11726496[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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