1d58: Difference between revisions

Revision as of 13:27, 2 May 2008

THE MOLECULAR STRUCTURE OF A 4'-EPIADRIAMYCIN COMPLEX WITH D(TGATCA) AT 1.7 ANGSTROM RESOLUTION-COMPARISON WITH THE STRUCTURE OF 4'-EPIADRIAMYCIN D(TGTACA) AND D(CGATCG) COMPLEXES

OverviewOverview

The structure of the complex between d(TGATCA) and the anthracycline 4'-epiadriamycin has been determined by crystallographic methods. The crystals are tetragonal, space group P4(1)2(1)2 with unit cell dimensions of a = 28.01, c = 52.95A. The asymmetric unit consists of one strand of hexanucleotide, one molecule of 4'-epiadriamycin and 34 waters. The R-factor is 20.2% for 1694 reflections with F greater than or equal to 2 sigma F to 1.7A. Two asymmetric units associate to generate a duplex complexed with two drug molecules at the d(TpG) steps of the duplex. The chromophore intercalates between these base pairs with the anthracycline amino-sugar positioned in the minor groove. The double helix is a distorted B-DNA type structure. Our structure determination of d(TGATCA) complexed to 4'-epiadriamycin allows for comparison with the previously reported structures of 4'-epiadriamycin bound to d(TGTACA) and to d(CGATCG). The three complexes are similar in gross features and the intercalation geometry is the same irrespective of whether a d(CpG) or d(TpG) sequence is involved. However, the orientation of the amino-sugar displays a dependence on the sequence adjacent to the intercalation site. The flexibility of this amino-sugar may help explain why this class of antibiotics displays a relative insensitivity to base sequence when they bind to DNA.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

The molecular structure of a 4'-epiadriamycin complex with d(TGATCA) at 1.7A resolution: comparison with the structure of 4'-epiadriamycin d(TGTACA) and d(CGATCG) complexes., Langlois d'Estaintot B, Gallois B, Brown T, Hunter WN, Nucleic Acids Res. 1992 Jul 25;20(14):3561-6. PMID:1641324 Page seeded by OCA on Fri May 2 13:27:54 2008

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OCA

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 [[Image:1d58.gif|left|200px]]
- {{Structure
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 '''THE MOLECULAR STRUCTURE OF A 4'-EPIADRIAMYCIN COMPLEX WITH D(TGATCA) AT 1.7 ANGSTROM RESOLUTION-COMPARISON WITH THE STRUCTURE OF 4'-EPIADRIAMYCIN D(TGTACA) AND D(CGATCG) COMPLEXES'''
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 ==About this Structure==
-D58 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D58 OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D58 OCA].
 ==Reference==
 The molecular structure of a 4'-epiadriamycin complex with d(TGATCA) at 1.7A resolution: comparison with the structure of 4'-epiadriamycin d(TGTACA) and d(CGATCG) complexes., Langlois d'Estaintot B, Gallois B, Brown T, Hunter WN, Nucleic Acids Res. 1992 Jul 25;20(14):3561-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1641324 1641324]
-[[Category: Protein complex]]
 [[Category: Brown, T.]]
 [[Category: Estaintot, B Langlois D.]]
 [[Category: Gallois, B.]]
 [[Category: Hunter, W N.]]
-[[Category: complexed with drug]]
+[[Category: Complexed with drug]]
-[[Category: double helix]]
+[[Category: Double helix]]
-[[Category: right handed dna]]
+[[Category: Right handed dna]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:27:54 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:34:57 2008''