1d39: Difference between revisions

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COVALENT MODIFICATION OF GUANINE BASES IN DOUBLE STRANDED DNA: THE 1.2 ANGSTROMS Z-DNA STRUCTURE OF D(CGCGCG) IN THE PRESENCE OF CUCL2

OverviewOverview

We have solved the single crystal structure to 1.2-A resolution of the Z-DNA sequence d(CGCGCG) soaked with copper(II) chloride. This structure allows us to elucidate the structural properties of copper in a model that mimics a physiologically relevant environment. A copper(II) cation was observed to form a covalent coordinate bond to N-7 of each guanine base along the hexamer duplex. The occurrence of copper bound at each site was dependent on the exposure of the bases and the packing of the hexamers in the crystal. The copper at the highest occupied site was observed to form a regular octahedral complex, with four water ligands in the equatorial plane and a fifth water along with N-7 of the purine base at the axial positions. All other copper complexes appear to be variations of this structure. By using the octahedral complex as the prototype for copper(II) binding to guanine bases in the Z-DNA crystal, model structures were built showing that duplex B-DNA can accommodate octahedral copper(II) complexes at the guanine bases as well as copper complexes bridged at adjacent guanine residues by a reactive dioxygen species. The increased susceptibility to oxidative DNA cleavage induced by copper(II) ions in solution of the bases located 5' to one or more adjacent guanine residues can thus be explained in terms of the cation and DNA structures described by these models.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Covalent modification of guanine bases in double-stranded DNA. The 1.2-A Z-DNA structure of d(CGCGCG) in the presence of CuCl2., Kagawa TF, Geierstanger BH, Wang AH, Ho PS, J Biol Chem. 1991 Oct 25;266(30):20175-84. PMID:1939078 Page seeded by OCA on Fri May 2 13:24:05 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1d39.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1d39 |SIZE=350|CAPTION= <scene name='initialview01'>1d39</scene>, resolution 1.200&Aring;
+The line below this paragraph, containing "STRUCTURE_1d39", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1d39|  PDB=1d39  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d39 OCA], [http://www.ebi.ac.uk/pdbsum/1d39 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d39 RCSB]</span>
-}}
 '''COVALENT MODIFICATION OF GUANINE BASES IN DOUBLE STRANDED DNA: THE 1.2 ANGSTROMS Z-DNA STRUCTURE OF D(CGCGCG) IN THE PRESENCE OF CUCL2'''
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 ==About this Structure==
-D39 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D39 OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D39 OCA].
 ==Reference==
 Covalent modification of guanine bases in double-stranded DNA. The 1.2-A Z-DNA structure of d(CGCGCG) in the presence of CuCl2., Kagawa TF, Geierstanger BH, Wang AH, Ho PS, J Biol Chem. 1991 Oct 25;266(30):20175-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1939078 1939078]
-[[Category: Protein complex]]
 [[Category: Geierstanger, B H.]]
 [[Category: Ho, P S.]]
 [[Category: Kagawa, T F.]]
 [[Category: Wang, A H.J.]]
-[[Category: double helix]]
+[[Category: Double helix]]
-[[Category: modified]]
+[[Category: Modified]]
-[[Category: z-dna]]
+[[Category: Z-dna]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:24:05 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:33:45 2008''