6ek8: Difference between revisions
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==YaxB from Yersinia enterocolitica== | ==YaxB from Yersinia enterocolitica== | ||
<StructureSection load='6ek8' size='340' side='right' caption='[[6ek8]], [[Resolution|resolution]] 4.00Å' scene=''> | <StructureSection load='6ek8' size='340' side='right'caption='[[6ek8]], [[Resolution|resolution]] 4.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6ek8]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EK8 OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[6ek8]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EK8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6EK8 FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6ek4|6ek4]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6ek4|6ek4]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ek8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ek8 OCA], [http://pdbe.org/6ek8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ek8 RCSB], [http://www.ebi.ac.uk/pdbsum/6ek8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ek8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Braeuning, B]] | [[Category: Braeuning, B]] | ||
[[Category: Groll, M]] | [[Category: Groll, M]] | ||
Latest revision as of 12:11, 11 November 2020
YaxB from Yersinia enterocoliticaYaxB from Yersinia enterocolitica
Structural highlights
Publication Abstract from PubMedPore-forming toxins (PFT) are virulence factors that transform from soluble to membrane-bound states. The Yersinia YaxAB system represents a family of binary alpha-PFTs with orthologues in human, insect, and plant pathogens, with unknown structures. YaxAB was shown to be cytotoxic and likely involved in pathogenesis, though the molecular basis for its two-component lytic mechanism remains elusive. Here, we present crystal structures of YaxA and YaxB, together with a cryo-electron microscopy map of the YaxAB complex. Our structures reveal a pore predominantly composed of decamers of YaxA-YaxB heterodimers. Both subunits bear membrane-active moieties, but only YaxA is capable of binding to membranes by itself. YaxB can subsequently be recruited to membrane-associated YaxA and induced to present its lytic transmembrane helices. Pore formation can progress by further oligomerization of YaxA-YaxB dimers. Our results allow for a comparison between pore assemblies belonging to the wider ClyA-like family of alpha-PFTs, highlighting diverse pore architectures. Structure and mechanism of the two-component alpha-helical pore-forming toxin YaxAB.,Brauning B, Bertosin E, Praetorius F, Ihling C, Schatt A, Adler A, Richter K, Sinz A, Dietz H, Groll M Nat Commun. 2018 May 4;9(1):1806. doi: 10.1038/s41467-018-04139-2. PMID:29728606[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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