6clc: Difference between revisions
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==1.01 A MicroED structure of GSNQNNF at 0.27 e- / A^2== | ==1.01 A MicroED structure of GSNQNNF at 0.27 e- / A^2== | ||
<StructureSection load='6clc' size='340' side='right' caption='[[6clc]], [[Resolution|resolution]] 1.01Å' scene=''> | <StructureSection load='6clc' size='340' side='right'caption='[[6clc]], [[Resolution|resolution]] 1.01Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6clc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CLC OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[6clc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CLC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6CLC FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6clc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6clc OCA], [http://pdbe.org/6clc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6clc RCSB], [http://www.ebi.ac.uk/pdbsum/6clc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6clc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Gallagher-Jones, M]] | [[Category: Gallagher-Jones, M]] | ||
[[Category: Glynn, C]] | [[Category: Glynn, C]] | ||
Revision as of 11:58, 11 November 2020
1.01 A MicroED structure of GSNQNNF at 0.27 e- / A^21.01 A MicroED structure of GSNQNNF at 0.27 e- / A^2
Structural highlights
Publication Abstract from PubMedMicro-crystal electron diffraction (MicroED) combines the efficiency of electron scattering with diffraction to allow structure determination from nano-sized crystalline samples in cryoelectron microscopy (cryo-EM). It has been used to solve structures of a diverse set of biomolecules and materials, in some cases to sub-atomic resolution. However, little is known about the damaging effects of the electron beam on samples during such measurements. We assess global and site-specific damage from electron radiation on nanocrystals of proteinase K and of a prion hepta-peptide and find that the dynamics of electron-induced damage follow well-established trends observed in X-ray crystallography. Metal ions are perturbed, disulfide bonds are broken, and acidic side chains are decarboxylated while the diffracted intensities decay exponentially with increasing exposure. A better understanding of radiation damage in MicroED improves our assessment and processing of all types of cryo-EM data. Analysis of Global and Site-Specific Radiation Damage in Cryo-EM.,Hattne J, Shi D, Glynn C, Zee CT, Gallagher-Jones M, Martynowycz MW, Rodriguez JA, Gonen T Structure. 2018 May 1;26(5):759-766.e4. doi: 10.1016/j.str.2018.03.021. Epub 2018, Apr 26. PMID:29706530[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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