4ugy: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Structure of Bacillus subtilis Nitric Oxide Synthase in complex with N1-(5-(2-(6-Amino-4-methylpyridin-2-yl)ethyl)pyridin-3-yl)-N1,N2- dimethylethane-1,2-diamine== | ==Structure of Bacillus subtilis Nitric Oxide Synthase in complex with N1-(5-(2-(6-Amino-4-methylpyridin-2-yl)ethyl)pyridin-3-yl)-N1,N2- dimethylethane-1,2-diamine== | ||
<StructureSection load='4ugy' size='340' side='right' caption='[[4ugy]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='4ugy' size='340' side='right'caption='[[4ugy]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ugy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UGY OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[4ugy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UGY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4UGY FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EXI:N1-(5-(2-(6-AMINO-4-METHYLPYRIDIN-2-YL)ETHYL)PYRIDIN-3-YL)-N1,N2-DIMETHYLETHANE-1,2-DIAMINE'>EXI</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EXI:N1-(5-(2-(6-AMINO-4-METHYLPYRIDIN-2-YL)ETHYL)PYRIDIN-3-YL)-N1,N2-DIMETHYLETHANE-1,2-DIAMINE'>EXI</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ug5|4ug5]], [[4ug6|4ug6]], [[4ug7|4ug7]], [[4ug8|4ug8]], [[4ug9|4ug9]], [[4uga|4uga]], [[4ugb|4ugb]], [[4ugc|4ugc]], [[4ugd|4ugd]], [[4uge|4uge]], [[4ugf|4ugf]], [[4ugg|4ugg]], [[4ugh|4ugh]], [[4ugi|4ugi]], [[4ugj|4ugj]], [[4ugk|4ugk]], [[4ugl|4ugl]], [[4ugm|4ugm]], [[4ugn|4ugn]], [[4ugo|4ugo]], [[4ugp|4ugp]], [[4ugq|4ugq]], [[4ugr|4ugr]], [[4ugs|4ugs]], [[4ugt|4ugt]], [[4ugu|4ugu]], [[4ugv|4ugv]], [[4ugw|4ugw]], [[4ugx|4ugx]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ug5|4ug5]], [[4ug6|4ug6]], [[4ug7|4ug7]], [[4ug8|4ug8]], [[4ug9|4ug9]], [[4uga|4uga]], [[4ugb|4ugb]], [[4ugc|4ugc]], [[4ugd|4ugd]], [[4uge|4uge]], [[4ugf|4ugf]], [[4ugg|4ugg]], [[4ugh|4ugh]], [[4ugi|4ugi]], [[4ugj|4ugj]], [[4ugk|4ugk]], [[4ugl|4ugl]], [[4ugm|4ugm]], [[4ugn|4ugn]], [[4ugo|4ugo]], [[4ugp|4ugp]], [[4ugq|4ugq]], [[4ugr|4ugr]], [[4ugs|4ugs]], [[4ugt|4ugt]], [[4ugu|4ugu]], [[4ugv|4ugv]], [[4ugw|4ugw]], [[4ugx|4ugx]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_synthase_(NAD(P)H-dependent) Nitric-oxide synthase (NAD(P)H-dependent)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.165 1.14.13.165] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_synthase_(NAD(P)H-dependent) Nitric-oxide synthase (NAD(P)H-dependent)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.165 1.14.13.165] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4ugy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ugy OCA], [http://pdbe.org/4ugy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ugy RCSB], [http://www.ebi.ac.uk/pdbsum/4ugy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ugy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| Line 22: | Line 22: | ||
==See Also== | ==See Also== | ||
*[[Nitric Oxide Synthase|Nitric Oxide Synthase]] | *[[Nitric Oxide Synthase 3D structures|Nitric Oxide Synthase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 28: | Line 28: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bacsu]] | [[Category: Bacsu]] | ||
[[Category: Large Structures]] | |||
[[Category: Holden, J K]] | [[Category: Holden, J K]] | ||
[[Category: Poulos, T L]] | [[Category: Poulos, T L]] | ||
[[Category: Inhibitor]] | [[Category: Inhibitor]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
Revision as of 11:21, 4 November 2020
Structure of Bacillus subtilis Nitric Oxide Synthase in complex with N1-(5-(2-(6-Amino-4-methylpyridin-2-yl)ethyl)pyridin-3-yl)-N1,N2- dimethylethane-1,2-diamineStructure of Bacillus subtilis Nitric Oxide Synthase in complex with N1-(5-(2-(6-Amino-4-methylpyridin-2-yl)ethyl)pyridin-3-yl)-N1,N2- dimethylethane-1,2-diamine
Structural highlights
Function[NOSO_BACSU] Catalyzes the production of nitric oxide. Publication Abstract from PubMedNitric oxide generated by bacterial nitric oxide synthase (NOS) increases the susceptibility of Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis to oxidative stress, including antibiotic-induced oxidative stress. Not surprisingly, NOS inhibitors also improve the effectiveness of antimicrobials. Development of potent and selective bacterial NOS inhibitors is complicated by the high active site sequence and structural conservation shared with the mammalian NOS isoforms. To exploit bacterial NOS for the development of new therapeutics, recognition of alternative NOS surfaces and pharmacophores suitable for drug binding is required. Here, we report on a wide number of inhibitor-bound bacterial NOS crystal structures to identify several compounds that interact with surfaces unique to the bacterial NOS. Although binding studies indicate that these inhibitors weakly interact with the NOS active site, many of the inhibitors reported here provide a revised structural framework for the development of new antimicrobials that target bacterial NOS. In addition, mutagenesis studies reveal several key residues that unlock access to bacterial NOS surfaces that could provide the selectivity required to develop potent bacterial NOS inhibitors. Inhibitor Bound Crystal Structures of Bacterial Nitric Oxide Synthase.,Holden JK, Dejam D, Lewis MC, Huang H, Kang S, Jing Q, Xue F, Silverman RB, Poulos TL Biochemistry. 2015 Jun 23. PMID:26062720[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||||